|Table of Contents|

CSD polypeptide inhibits the invasion and migration of ovarian cancer SKOV3 cell

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 02
Page:
199-204
Research Field:
Publishing date:

Info

Title:
CSD polypeptide inhibits the invasion and migration of ovarian cancer SKOV3 cell
Author(s):
ZHENG Qingling1; ZHANG Ting1; ZHANG Mingmei1; GU Donghua2
1.Medicine School of Huzhou University,Zhejiang Huzhou 313000,China;2.Department of Pathology,Affiliated Suzhou Science & Technology Town Hospital of Nanjing Medical University,Jiangsu Suzhou 215153,China.
Keywords:
caveolin-1; ovarian neoplasm; invasion; polypeptide
PACS:
R737.31
DOI:
10.3969/j.issn.1672-4992.2022.02.004
Abstract:
Objective：To study the effect of caveolin-1 scaffolding domain(CSD) polypeptide on the ability of migration and invasion in SKOV3 cell，and to explore its molecular mechanism．Methods:Immunocytofluorescence was applied to observe the infiltrability of CSD polypeptide in SKOV3 cell.The scratch test was applied to test the migratory ability,and Transwell in vitro invasion assay was used for the invasive ability of SKOV3 cell.E-cadherin and Vimentin protein expression were evaluated by Western blot and Immunocytofluorescence.EGFR,PI3K and Akt protein expresion and their relative phosphorylation levels were detected by Western blot.Results:Immunocytofluorescence results showed CSD polypeptide can be detected in cytoplasm of SKOV3 cell.Treated with CSD polypeptide,the ability of invasion and migration were significantly inhibited compare with blank control group（P＜0.01）.Compared with blank control group,CSD polypeptide can upregulate E-cadherin protein level （P＜0.01） and inhibite Vimentin protein expression（P＜0.01） in SKOV3 cell.According to Western blot results，the relative phosphorylation levels of EGFR，PI3K and Akt in CSD polypeptide group were significantly lower than that in blank control group（P＜0.01,P＜0.05,P＜0.05).Conclusion:caveolin-1 scaffolding domain polypeptide is able to inhibit the migratory and invasive ability of SKOV3 cell and reverse the epithelial-mesenchymal transition,EGFR/PI3K/Akt signaling pathway may be involved in its molecular mechanisms.

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Memo

Memo:
浙江省湖州市自然科学资金（编号:2020YZ03）；湖州师范学院校级科研项目（编号:2020XJKJ10）
Last Update: 1900-01-01