|Table of Contents|

Total flavonoids extract from Sculellaria barbata D.Don inhibits the proliferation,migration and invasion of cervical cancer cells by regulating lncRNA LINC00858/miR-324-5p pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 01
Page:
38-44
Research Field:
Publishing date:

Info

Title:
Total flavonoids extract from Sculellaria barbata D.Don inhibits the proliferation,migration and invasion of cervical cancer cells by regulating lncRNA LINC00858/miR-324-5p pathway
Author(s):
XU Lei1; WANG Shanshan2; CHEN Lin1
1.Obstetrics Department；2.Health Care Section，Shiyan Maternal and Child Health Hospital，Hubei Shiyan 442000,China.
Keywords:
total flavonoids extract from Scutellaria barbata D.Don; LINC00858; miR-324-5p; cervical cancer; proliferation; invasion; migration
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2022.01.008
Abstract:
Objective:To investigate the effect of total flavonoids extract from Sculellaria barbata D.Don(TFESB) on the proliferation,migration and invasion of cervical cancer cells by regulating lncRNA LINC00858/miR-324-5p pathway.Methods:qRT-PCR was used to detect the expression levels of LINC00858 and miR-324-5p in 20 cervical cancer tissues and their corresponding adjacent tissues.SiHa cells were randomly divided into Con group,TFESB treatment group,si-NC group,si-LINC00858 group,miR-NC group,miR-324-5p group,TFESB+pcDNA group and TFESB+LINC00858 group.The expression levels of LINC00858 and miR-324-5p in each group of SiHa cells were detected by qRT-PCR.The cell proliferation activity was measured by the CCK-8 method.Transwell assay was used to detect cell migration and invasion ability.The expression levels of proliferation-associated proteins(CyclinD1,p21) and migration-invasive related proteins(MMP-2,MMP-9) were detected by Western blot.The dual luciferase reporter assay and qRT-PCR were to verify the targeted and regulatory relationship between LINC00858 and miR-324-5p.Results:Compared with adjacent tissues,the expression level of LINC00858 in cervical cancer tissues was significantly increased,while the expression level of miR-324-5p was significantly decreased.Compared with the Con group,the expression level of LINC00858 in SiHa cells treated with TFESB was significantly decreased,the expression level of miR-324-5p was significantly increased,the proliferation,migration and invasion ability of the cells were significantly decreased,and showed a significant concentration-dependent.Compared with the si-NC group,the proliferation,migration and invasion ability of SiHa cells in the si-LINC00858 group were significantly decreased.Compared with the miR-NC group,the proliferation,migration and invasion ability of SiHa cells in the miR-324-5p group were significantly reduced.Compared with the TFESB+pcDNA group,the proliferation,migration and invasion ability of SiHa cells in the TFESB+pcDNA-LINC00858 group were significantly increased.Conclusion:The total flavonoid extract from Sculellaria barbata D.Don.inhibits the proliferation,migration and invasion of cervical cancer cells,and its mechanism is related to the regulation of lncRNA LINC00858/miR-324-5p pathway.

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Last Update: 2021-12-02