|Table of Contents|

The expression characteristic of inhibitory B7 family molecules in acute myeloid leukemia

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 05
Page:
872-877
Research Field:
Publishing date:

Info

Title:
The expression characteristic of inhibitory B7 family molecules in acute myeloid leukemia
Author(s):
ZHANG Lingyi1; 2; ZHANG Wei3; LIN Jiang2; CHEN Qiaoyun2; QIAN Jun3; YUAN Qian3; ZHOU Jingdong3; ZHANG Tingjuan1; 3; ZHOU Hong1
1.School of Medicine,Jiangsu University,Jiangsu Zhenjiang 212013,China;2.Laboratory Center;3Department of Hematology,the Affiliated People's Hospital of Jiangsu University,Jiangsu Zhenjiang 212002,China.
Keywords:
B7 costimulatory molecules; flow cytometry; acute myeloid leukemia; membrane protein; expression
PACS:
R733.71
DOI:
10.3969/j.issn.1672-4992.2021.05.034
Abstract:
Objective:To investigate the expression and distribution of B7-H1,B7-H3,B7-H4 and B7-H6 in human acute myeloid leukemia (AML).Methods:The membrane protein expression of B7-H1,B7-H3,B7-H4 and B7-H6 in 8 AML cell lines and bone marrow blast cells of 22 de novo AML patients were detected by flow cytometry,with that of 8 volunteers as control.The expression rate of positive cells,mean fluorescence intensity (MFI) and mean fluorescence intensity ratio of blast cells and lymphocytes (MFI ratio) were used to evaluate the expression characteristic of these B7 molecules.Results:Majority of AML cell lines expressed high or detectable level of membrane protein B7-H3 and B7-H6.The expression of B7-H3 was higher in SKM-1 and SHI-1,and B7-H6 had higher expression in K562 and HL-60.B7-H1 was highly expressed only in HEL,while B7-H1 membrane protein was not expressed in other AML cell lines.B7-H4 was undetectable in AML cell lines.The expression rate and MFI ratio of membrane protein B7-H3 and B7-H6 in de novo AML blast cells was significantly higher than that of control.And peripheral white blood cells of group with high B7-H3 expression were significantly higher when compared with group with low B7-H3 expression,and age of the group with high B7-H6 expression was also significantly higher than that of the group with low B7-H6 expression.Expression of B7-H3 and B7-H6 was elevated in patients with acute monocytic leukemia.The expression of B7-H1 and B7-H4 in de novo AML patients was low or undetectable,and there was no significant difference compared with control.Conclusion:There are differences among expression characteristics of four inhibitory B7 family molecules in AML cell lines and patients.The abnormally elevated expression of B7-H3 and B7-H6 in de novo AML suggests that the two B7 family molecules may be involved in occurrence and progression of acute myeloid leukemia.

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Memo

Memo:
江苏省基础研究计划青年基金资助项目（编号：BK20180280）；镇江市社会发展项目（编号：SH2016045）；江苏大学附属人民医院博士启动基金资助项目（编号：KFB201603）
Last Update: 2021-01-29