|Table of Contents|

Bioinformatics analysis of miR-497 and its expression in lung adenocarcinoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 05
Page:
801-806
Research Field:
Publishing date:

Info

Title:
Bioinformatics analysis of miR-497 and its expression in lung adenocarcinoma
Author(s):
HU Wenhua1; FAN Huan2; CHEN Ting3; WANG Yongcun4; LIU Shuguang2
1.Department of Pathology;3.Clinical Research Center;4Department of Oncology,Affiliated Hospital of Guangdong Medical University,Guangdong Zhanjiang 524000,China;2.Department of Pathology,the Eighth Affiliated Hospital,Sun Yat-sen University,Guangdong Shenzhen 518000,China.
Keywords:
miR-497; target genes; gene function annotation; signaling pathway; bioinformatics; lung adenocarcinoma
PACS:
R734.2
DOI:
DOI:10.3969/j.issn.1672-4992.2021.05.018
Abstract:
Objective:To predict the target genes and functional bioinformatics analysis of miR-497,and provide theoretical guidance for further research on the biological function and regulatory mechanism of miR-497.Furthermore,its expression in lung adenocarcinoma was verified.Methods:Using PubMed to retrieve the relevant articles of miR-497,analyze its base sequence,chromosome location and other information through miRBase online tool,and analyze its conservative nature.Then,targetscan and miRDB were applied to predict the intersection of the target gene of miR-497.Finally,the target gene function enrichment and signal transduction pathway enrichment analysis were performed by using the confirmed target gene,the expression of miR-497 in fresh lung cancer and adjacent tissues was quantitatively detected by RT-PCR.Results:The results showed that the miR-497 was highly conservative among multiple species.The target gene function of has-miR-497 was mainly focused on ureteric bud development,transmembrane receprot protein kinase,peptide binding,morphogenesis of a branching and membrane raft (P＜0.05).KEGG biological pathway was mainly concentrated on the interaction pathway of regulation of stem cell pluripotent,pathways in cancer,Wnt and Rap1 signaling pathway (P＜0.001).The expression of miR-497 in lung cancer tissues was significantly lower than that in adjacent tissues (P＜0.05).Conclusion:miR-497 regulates multiple biological processes and disease pathways through target gene,especially in the direction of tumor.Lower expression of miR-497 in lung adenocarcinoma provides a new direction for predicting the occurrence,development and treatment of lung cancer.

References:

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Memo

Memo:
深圳市福田区卫生公益性科研项目（编号：FTWS2018043，FTWS2018047）
Last Update: 2021-01-29