|Table of Contents|

Regulation of miR-526b-3p on proliferation,migration and invasion of the liver cancercell by targeting TNKS2 gene

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 05
Page:
755-760
Research Field:
Publishing date:

Info

Title:
Regulation of miR-526b-3p on proliferation,migration and invasion of the liver cancercell by targeting TNKS2 gene
Author(s):
ZHAO Kechang; WU Shile; LIU Linxun; FENG Pengcai; YANG Jinyu
Department of General Surgery,Qinghai Provincial People's Hospital,Qinghai Xining 810007,China.
Keywords:
liver cancer; miR-526b-3p; TNKS2; cell proliferation; migration; invasion
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2021.05.008
Abstract:
Objective:To explore the effect of miR-526b-3p on proliferation,migration and invasion of liver cancer cell and its potential mechanism.Methods:Expressions of miR-526b-3p in liver cancer HepG2，SMMC-7721 and BEL-7402 cells as well as normal liver cell L02 were detected by qRT-PCR.The HepG2 cell line with overexpression of miR-526b-3p and the HepG2 cell line with knockdown of TNKS2 were constructed.MTT,Transwell and Western blot assays were used to measure the proliferation viability,the migration and invasion cell numbers and the expression of TNKS2 protein,respectively.Possible target gene of miR-526b-3p was verified with dual luciferase report gene assay.Results:Compared with normal liver cell L02,the expression of miR-526b-3p in liver cancer cell HepG2 was significantly decreased(P＜0.05),and the expression of TNKS2 was significantly increased（P＜0.05).The overexpression of miR-526b-3p and the knockdown of TNKS2 both inhibited the proliferation,migration and invasion of HepG2 cell（P＜0.05).Effect of miR-526b-3p on proliferation,migration and invasion of the HepG2 cell can be partially reversed by overexpression of TNKS2.Conclusion:miR-526b-3p can inhibit the proliferation,migration and invasion of liver cancer cells by down-regulating the expression of TNKS2.

References:

[1] TORRE LA,BRAY F,SIEGEL RL,et al.Global cancer statistics,2012［J］.CA Cancer J Clin,2015,65(2):87-108.
[2] FAN C,YARRAVARAPU N,CHEN H,et al.Regulation of tankyrase activity by a catalytic domain dimer interface［J］.Biochem Biophys Res Commun,2018,503(3):1780-1785.
[3] 李根,赵栋,李健,等.端锚聚合酶作为药物靶点的研究进展［J］.中国药理学通报,2018,34(09):1206-1210. LI G,ZHAO D,LI J,et al.Research progress of TNKS as drug target［J］.Chin Pharmacol Bull,2018,34(09):1206-1210.
[4] THORVALDSEN TE.Targeting tankyrase to fight WNT-dependent tumours［J］.Basic Clin Pharmacol Toxicol,2017,121(2):81-88.
[5] BUSCH AM,JOHNSON KC,STAN RV,et al.Evidence for tankyrases asantineoplastic targets in lung cancer［J］.BMC Cancer,2013,13(2):211-217.
[6] FAN QH,YU R,HUANG WX,et al.The has-miR-526b binding-site rs8506G>:a polymorphism in the lincRNA-NR-024015 exon identified by GWASs predispose to non-cardia gastric cancer risk［J］.PLoS One,2014,9(3):90008-90013.
[7] ZHANG ZY,FU SL,XU SQ,et al.By downregulating Ku80,hsa-miR-526b suppresses non-small cell lung cancer［J］.Oncotarget,2015,6(3):1462-1477.
[8] ZHANG R,ZHAO J,XU J,et al.miR-526b-3p functions as a tumor suppressor in colon cancer by regulating HIF-1α［J］.Am J Transl Res,2016,8(6):2783-2789.
[9] XIN L,LIN Y,JIAN FT,et al.MicroRNA-526b servers as a prognostic factor and exhibits tumor suppressive property by targeting Sirtuin 7 in hepatocellular carcinoma［J］.Oncotarget,2017,8(50):87737-87749.
[10] MAJUMDER M,LANDMAN E,LIU L,et al.COX-2 elevates oncogenic miR-526b in breast cancer by EP4 activation［J］.Mol Cancer Res,2015,13(6):1022-1033.
[11] LIN S,GREGORY RI.MicroRNA biogenesis pathways in cancer［J］.Nat Rev Cancer,2015,15(6):321-333.
[12] KIM MK，SMITH S.Persistent telomere cohesion triggers a prolonged anaphase［J］.Mol Biol Cell,2014,25(1):30-40．
[13] 段学英,杜二球,邰云燕.微小RNA-20a对宫颈癌细胞迁移、侵袭、增殖及ATG7表达的影响［J］.临床肿瘤学杂志,2018,196(04):28-33. DUAN XY,DU EQ,TAI YY.Effect of miRNA-20a on migration,invasion,proliferation and ATG7 expression of cervical cancer cells［J］.Journal of Clinical Oncology,2018,196(04):28-33.
[14] LU ML,ZHANG Y,LI J,et al.MicroRNA-124 inhibits colorectal cancer cell proliferation and suppresses tumor growth by interacting with PLCB1 and regulating Wnt/β-catenin signaling pathway［J］.Eur Rev Med Pharmacol Sci,2019,23（1):121-136.
[15] KIM MK.Novel insight into the function oftankyrase［J］.Oncol Lett,2018,16(6):6895-6902.
[16] MA L,WANG X,JIA T,et al.Tankyrase inhibitors attenuate WNT/β-catenin signaling and inhibit growth of hepatocellular carcinoma cells［J］.Oncotarget,2015,6(28):25390-25401.

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Last Update: 2021-01-29