|Table of Contents|

miR-182-5p inhibits angiogenesis by targeting Tiam1 in colorectal cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 05
Page:
749-754
Research Field:
Publishing date:

Info

Title:
miR-182-5p inhibits angiogenesis by targeting Tiam1 in colorectal cancer
Author(s):
CHEN Guoqun1; ZHOU Yadong1; CHEN Xiang2
1.Department of Pathology,the Fourth Hospital of Changsha,Hunan Changsha 410006,China;2.Department of Pathology,the Eighth Hospital of Changsha,Hunan Changsha 410100,China.
Keywords:
colorectal cancer; miR-182-5p; Tiam1; angiogenesis
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2021.05.007
Abstract:
Objective:To investigate whether miR-182-5p can inhibit angiogenesis by targeting Tiam1 in colorectal cancer.Methods:Human normal colonic epithelial cells and colorectal cancer cells were selected and the expression of miR-182-5p and Tiam1 mRNA in the cell line was detected by qPCR.The control mimics overexpression vector,miR-182-5p mimics overexpression vector,siRNA control overexpression vector,Tiam1 siRNA overexpression vector and Tiam1 overexpression vector were transfected into HT-29 cells.The targeting relationship between miR-182-5p and Tiam1 3' UTR was verified by luciferase reporter assay.HUVEC,cultured in the supernatant of engineering tumor cells was used to detect the ability of cell migration and tubule formation by wound-healing assay and tube formation assay,respectively.The expression of related proteins was detected by Western blot,and angiogenesis was detected by tumor xenograft model in vivo.Results:The results showed that the expression of miR-182-5p was significantly reduced in HT-29 cells（P＜0.01）,while the expression of Tiam1 was significantly improved in HT-29（P＜0.001）,and Tiam1 was the target of miR-182-5p.The results of migration assay showed that the supernatant of HT-29 cells transfected with miR-182-5p mimics could inhibit the migration ability of HUVEC（P＜0.01）,and the supernatant of HT-29 cells silenced by Tiam1 gene could also inhibit the migration ability of HUVEC（P＜0.001）.Tubule formation and in vivo experiments showed that overexpression miR-182-5p and inhibition of Tiam1 can effectively inhibit tumor angiogenesis（P＜0.01）.Conclusion:miR-182-5p can target Tiam1,and inhibit angiogenesis in colorectal cancer by inhibiting the expression of Tiam1.

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Memo

Memo:
湖南省卫生计生委课题（编号：B2017152）；长沙市科技局基础研究项目（编号：kq1907009）
Last Update: 2021-01-29