|Table of Contents|

LINC01089 inhibits the proliferation and migration of pancreatic cancer cells by regulating miR-27a-3p/TET1 axis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 05
Page:
732-738
Research Field:
Publishing date:

Info

Title:
LINC01089 inhibits the proliferation and migration of pancreatic cancer cells by regulating miR-27a-3p/TET1 axis
Author(s):
BAI Kunpeng1; CAO Juchao1; 2; JIANG Yiheng1
1.Department of Emergency,the Second People's Hospital of Guangdong,Guangdong Guangzhou 510000,China;2.Department of Emergency,the First People's Hospital of Kashgar,Xinjiang Kashgar 840000,China.
Keywords:
pancreatic cancer; LINC01089; miR-27a-3p; TET1; proliferation; migration
PACS:
R735.9
DOI:
10.3969/j.issn.1672-4992.2021.05.004
Abstract:
Objective:To investigate the role and mechanism of long non-coding RNA 01089（LINC01089)/miR-27a-3p/ten-eleven translocation 1(TET1) axis in pancreatic cancer progression.Methods:We analyzed the expression of LINC01089 in pancreatic cancer and its prognosis by GEPIA database.We used quantitative real-time polymerase chain reaction（qRT-PCR) to detect the expression of LINC01089,miR-27a-3p,TET1 mRNA in pancreatic cancer tissues and normal tissues.CCK-8,BrdU and scratch healing experiments were used to detect the effects of LINC01089 and miR-27a-3p on cell proliferation and migration.Mechanistically,we used StarBase and TargetScan database,the double luciferase reporter gene experiment to predict and verify the target relationship between LINC01089 and miR-27a-3p,miR-27a-3p and TET1.The effects of LINC01089 and miR-27a-3p on TET1 expression were detected by Western blot.Results:The GEPIA showed that LINC01089 was underexpressed in pancreatic cancer and correlated with patients' overall survival（OS) and diease free survival（DFS).In this study,we found that LINC01089 expression was significantly down-regulated in cancer tissues compared with normal tissues and was associated with poor prognosis of patients.Functional experiments showed that LINC01089 inhibited the proliferation and migration of pancreatic cancer cells.Relevant mechanism experiments showed that LINC01089 upregulated the expression of TET1 by adsorbing miR-27a-3p and played an inhibitory role in pancreatic cancer.Conclusion:LINC01089 promotes TET1 expression by targeting and regulating miR-27a-3p,thereby inhibiting the proliferation and migration of pancreatic cancer.

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广东省援疆农村科技项目资助（编号：KTP20190286）
Last Update: 2021-01-29