|Table of Contents|

Aloe-emodin nanoliposome-mediated photodynamic therapy inhibits proliferation and promotes autophagy of human oral cancer CAL-27 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 05
Page:
719-723
Research Field:
Publishing date:

Info

Title:
Aloe-emodin nanoliposome-mediated photodynamic therapy inhibits proliferation and promotes autophagy of human oral cancer CAL-27 cells
Author(s):
WANG He; DONG Li; SHAO Mingjing; ZHAO Lei; SUN Xiaodong; MENG Peisong
Department of Stomatology,the Fourth Medical College of Harbin Medical University,Heilongjiang Harbin 150001,China.
Keywords:
aloe-emodin; liposomes; photodynamic therapy; oral cance; autophagy
PACS:
R739.85
DOI:
10.3969/j.issn.1672-4992.2021.05.001
Abstract:
Objective:To investigate the effect of photodynamic therapy(PDT) mediated by aloe-emodin nanoliposome（AE-L) on human oral cancer CAL-27 cells.Methods:AE-L was prepared by inverse evaporation.The effect of AE-L(0,2.5,5.0,7.5,10.0,12.5 μg/mL) or 405 nm laser(power density at 80 mW/cm2,duration of 0,10,20,40,80,160 s)on the survival rate of CAL-27 cells was analyzed quantitatively by MTT assay.The effects of AE-L and 405 nm laser on the survival rate of CAL-27 cells were detected by MTT method.The uptake of AE-L（10 μg/mL) by CAL-27 cells was observed with fluorescence microscope.The effect of PDT（405 nm laser,power density at 80 mW/cm2,duration of 0,10,20,40,80,160 s) on the survival rate of CAL-27 cells was analyzed quantitatively by MTT assay.The formation of autophagosomes in CAL-27 cells was detected by using the monodansylcadaverine(MDC) and transmission electron microscopy.Results:The uptake of AE-L by CAL-27 cells reached the maximum at the sixth hour,which was the best time for laser intervention.When the concentrations of AE-L are less than 10 μg/mL,the survival rate of CAL-27 cells was not significantly affected(P＞0.05).The effect of 405 nm laser irradiation time on the survival rate of CAL-27 was not significantly different(P＞0.05).After treated with the PDT mediated by AE-L,and CAL-27 cells was inhibited in a light-time-dependent manner（P＜0.05).Compared with the control group,a large number of autophagosomes were showed in CAL-27 cells of the PDT group.Conclusion:The PDT mediated by AE-L could significantly suppress the proliferation of oral cancer CAL-27 cells and could promote autophagy of oral cancer CAL-27cells.

References:

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Memo

Memo:
哈尔滨医科大学附属第四医院特别资助项目(编号：HYDSYTB201915)
Last Update: 2021-01-29