|Table of Contents|

Expression of miR-194-5p and its correlation with MEX3a in small cell lung cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 04
Page:
585-589
Research Field:
Publishing date:

Info

Title:
Expression of miR-194-5p and its correlation with MEX3a in small cell lung cancer
Author(s):
LIU Baolin1; LI Aixin1; GU Qingfeng1; XU Jia1; ZHANG Zhiming1; ZU Haiying1; ZHANG Hongjie1; WU Ruifeng2; DOU Xuejun3; TAN Fengwei4
1.Department of General Surgery,Yixian Hospital,Hebei Baoding 074200,China;2.Department of Thoracic Surgery,Baoding First Central Hospital,Hebei Baoding 070000,China;3.Department of Thoracic Surgery,Beijing Space Center Hospital，Beijing 100000,China;4.Ca
Keywords:
lung; small cell carcinoma; miR-194-5p; MEX3a; prognosis
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2021.04.009
Abstract:
Objective：To detect the expression of microRNA-194-5p (miR-194-5p) in small cell lung cancer,explore its clinical significance,pay attention to its relationship with MEX3a.Methods:51 patients with small cell lung cancer were selected as the observation group.10 patients with large cell carcinoma,10 patients with typical carcinoid and 5 patients with atypical carcinoid were selected as the control group.Expression of miR-194-5p was detected by real-time fluorescence quantitative PCR in two groups.Expression of MEX3a was detected by immunohistochemistry in the observation group.Results:Expression of miR-194-5p was higher in the observation group than that in large cell carcinoma.Expression of miR-194-5p was lower in the observation group than that in typical carcinoid and atypical carcinoid.Expression of miR-194-5p was significantly different in tumor maximum diameter and proliferation index,but not in gender,age,location and lesions number in the observation group.Expression of miR-194-5p was related to survival time.Negative correlation was found between miR-194-5p and MEX3a.Conclusion:Abnormal expression of miR-194-5p is related to the progress of the disease in small cell lung cancer.miR-194-5p and MEX3a have negative correlation.miR-194-5p may have relationship with prognosis in small cell lung cancer.

References:

［1］ SETHI T,RINTOUL RC,MOORE SM,et al.Extracellular matrix proteins protect small cell lung cancer cells against apoptosis:A mechanism for small cell lung cancer growth and drug resistance in vivo［J］.Nat Med，1999,5(6):662-668.
[2] JIANG H,ZHANG X,LUO J,et al.Knockdown of hMex-3A by small RNA interference suppresses cell proliferation and migration in human gastric cancer cells［J］.Mol Med Rep,2012,6(3):575-580.
[3] BUCHET-POYAU K,COURCHET J,LE HIR H,et al.Identification and characterization of human Mex-3 proteins,a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies［J］.Nucleic Acids Res,2007,35(4):1289-1300.
[4] HE Y,DENG F,ZHAO S,et al.Analysis of miRNA-mRNA network reveals miR-140-5p as a suppressor of breast cancer glycolysis via targeting GLUT1［J］.Epigenomics,2019,11(9):1021-1036.
[5] WILLIAM D TRAVIS,ELIZABETH BRAMBILLA,H KONRAD MULLER-HERMELINK.Pathology and genetics tumour of the lung,pleura,thymus and heart［M］.Lyon：IARC Press,2006:27-31.
[6] FERNANDEZ-BARRAL A,COSTALES-CARRERA A,BUIRA SP,et al.Vitamin D differentially regulates colon stem cells in patient-derived normal and tumor organoids［J］.FEBS J,2020,287(1):53-72.
[7] CHATTERJI P,RUSTGI AK.RNA binding proteins in intestinal epithelial biology and colorectal cancer［J］.Trends Mol Med,2018,24(5):490-506.
[8] SHI JW,HUANG Y.Mex3a expression and survival analysis of bladder urothelial carcinoma［J］.Oncotarget,2017,8(33):54764-54774.
[9] BARRIGA FM,MONTAGNI E,MANA M,et al.Mex3a marks a slowly dividing subpopulation of Lgr5+ intestinal stem cells［J］.Cell Stem Cell,2017,20(6):801-816.
[10] LI CF,LI YC,WANG Y,et al.The effect of lncRNA H19/miR-194-5p axis on the epithelial-mesenchymal transition of colorectal adenocarcinoma［J］.Cell Physiol Biochem,2018,50(1):196-213.
[11] JUNG DE,PARK SB,KIM K,et al.CG200745,an HDAC inhibitor,induces anti-tumour effects in cholangiocarcinoma cell lines via miRNAs targeting the Hippo pathway［J］.Sci Rep,2017,7(1):10921-10922.
[12] YE Y,ZHANG F,CHEN Q,et al.LncRNA MALAT1 modified progression of clear cell kidney carcinoma (KIRC) by regulation of miR-194-5p/ACVR2B signaling［J］.Mol Carcinog,2019,58(2):279-292.
[13] JIANG Y,GAO H,LIU M,et al.Sorting and biological characteristics analysis for side population cells in human primary hepatocellular carcinoma［J］.Am J Cancer Res,2016,6(9):1890-1905.
[14] YUMIOKA T,OSAKI M,SASAKI R,et al.Lysosome-associated membrane protein 2 (LAMP-2) expression induced by miR-194-5p downregulation contributes to sunitinib resistance in human renal cell carcinoma cells［J］.Oncol Lett,2018,15(1):893-900.
[15] KONG Q,ZHANG S,LIANG C,et al.LncRNA XIST functions as a molecular sponge of miR-194-5p to regulate MAPK1 expression in hepatocellular carcinoma cell［J］.J Cell Biochem,2018,119(6):4458-4468.
[16] WANG Y,YANG L,CHEN T,et al.A novel lncRNA MCM3AP-AS1 promotes the growth of hepatocellular carcinoma by targeting miR-194-5p/FOXA1 axis［J］.Mol Cancer,2019,18(1):28-29.
[17] 刘占伟,祖恩霞,潘跃银,等.miR-194-5p靶向MEX3A调控肝癌细胞活性和凋亡的机制［J］.中国老年学杂志,2019,39(18):4551-4553. LIU ZW,ZU EX,PAN YY,et al.Mechanism of miＲ-194-5p regulating cell activity and apoptosis of hepatic carcinoma by targeting MEX3A［J］.Chin J Gerontol,2019,39(18):4551-4553.
[18] PEREIRA B,SOUSA S,BARROS R,et al.CDX2 regulation by the RNA-binding protein MEX3A:impact on intestinal differentiation and stemness［J］.Nucleic Acids Res,2013,41(7):3986-3999.

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