|Table of Contents|

miR-203a-3p enhances radiosensitivity of osteosarcoma cells by targeting AKT2

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 04
Page:
559-564
Research Field:
Publishing date:

Info

Title:
miR-203a-3p enhances radiosensitivity of osteosarcoma cells by targeting AKT2
Author(s):
ZHANG Junchang; XU Bin
Department of Orthopaedics,the First Hospital of Shanxi Medical University,Shanxi Taiyuan 030001,China.
Keywords:
osteosarcoma; MG-63; miR-203a-3p; AKT2; radiosensitivity; apoptosis
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2021.04.004
Abstract:
Objective：To investigate the effect of miR-203a-3p on apoptosis and radiosensitivity of osteosarcoma MG-63 cells and its potential mechanism.Methods:qRT-PCR was used to detect the expression level of AKT2 mRNA and the expression of miR-203a-3p in MG-63 cells treated with different doses of radiation (0,2,4,6,8 Gy).Colony formation assay was carried out to measure the survival fraction of MG-63 cells treated with different doses of radiation.Flow cytometry was used to detect the apoptotic rate of MG-63 cells.Western blot was applied to detect the expression level of AKT2 protein.And dual-luciferase reporter assay system was implemented to verify the relationship between miR-203a-3p and AKT2.Results:With the increase of radiation dose,the expression of miR-203a-3p in osteosarcoma MG-63 cells was significantly inhibited (P＜0.05).Overexpression of miR-203a-3p enhanced the radiosensitivity and promoted the apoptosis of MG-63 cells.The results of dual-luciferase reporter assay system suggested that miR-203a-3p targeted and negatively regulated the expression of AKT2.Inhibition of AKT2 enhanced the radiosensitivity of osteosarcoma MG-63 cells.Overexpression of AKT2 reversed the radiosensitization of MG-63 cells when miR-203a-3p was up-regulated and inhibited the apoptosis of MG-63 cells.Conclusion:miR-203a-3p enhances radiosensitivity and promotes apoptosis of osteosarcoma MG-63 cells by targeting AKT2.miR-203a-3p is expected to be a target for clinical radiosensitization of osteosarcoma.

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