|Table of Contents|

Targeted inhibition of miR-155 increases the sensitivity of myeloma cells to chemotherapeutic drugs

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 04
Page:
548-552
Research Field:
Publishing date:

Info

Title:
Targeted inhibition of miR-155 increases the sensitivity of myeloma cells to chemotherapeutic drugs
Author(s):
LI Lan1; GAO Ying1; ZHENG Yan1; ZHANG Weihua1; 2; MIAO Yudi1
1.Department of Hematology;2.Central Laboratory,Shaanxi Provincial People's Hospital，Shaanxi Xi'an 710068,China.
Keywords:
miR-155; myeloma; drug resistance; E-cadherin protein
PACS:
R733.3
DOI:
10.3969/j.issn.1672-4992.2021.04.002
Abstract:
Objective：To investigate the effect of targeted inhibition of miR-155 on chemotherapy sensitivity of myeloma cells and its molecular mechanism.Methods:The expression of miR-155 in myeloma cell line and drug resistant line was measured by qRT-PCR.LipofectamineTM 2000 was used to transfer miR-155 inhibitor and negative control into human myeloma resistant cell line.MTT assay was used to detect the sensitivity to chemotherapeutic drugs and proliferative activity after transfection of human myeloma drug-resistant cells.The expression of E-cadherin protein in human myeloma drug-resistant cells after transfection was determined by Western-blot assay.Results:The result of qRT-PCR showed that the expression level of miR-155 in human myeloma drug-resistant cells was significantly higher than that of human myeloma cells(P＜0.001).The results of MTT showed that the expression of miR-155 was inhibited by the transfection of miR-155 inhibitor.The sensitivity of human myeloma drug-resistant cells to chemotherapy drugs was significantly increased(P＜0.05).The cell proliferation activity was significantly decreased(P＜0.05).The result of Western-blot showed that the expression of E-cadherin protein in human myeloma-resistant cells increased significantly(P＜0.01).Conclusion:Targeted inhibition of miR-155 expression can increase the sensitivity of myeloma cells to chemotherapeutic drugs,and the mechanism may be related to the reversal of epithelial mesenchymal transition(EMT)emergence.

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Memo

Memo:
陕西省社会发展科技攻关项目（编号：2015SF065）;陕西省自然科学研究项目（编号：18PJ096）
Last Update: 1900-01-01