|Table of Contents|

miR-338-3p inhibits cell migration and invasion in gastric cancer cells by targeting PTP1B

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 04
Page:
543-547
Research Field:
Publishing date:

Info

Title:
miR-338-3p inhibits cell migration and invasion in gastric cancer cells by targeting PTP1B
Author(s):
WANG Qiwei; ZHANG Jingdong
Medical Oncology Department of Gastrointestinal Cancer，Liaoning Cancer Hospital ＆ Institute，Liaoning Shenyang 110042,China.
Keywords:
miR-338-3p; PTP1B; gastric cancer; migration; invasion
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2021.04.001
Abstract:
Objective：To investigate the effects of miR-338-3p and PTP1B on the migration and invasion of gastric cancer cells.Methods:Western Blot was used to test the expression of PTP1B in gastric cancer specimens and adjacent tissues.qRT-PCR was used to detect the expression of miR-338-3p.SGC7901 cells were transfected by PTP1B plasmid and siRNA,miR-338-3p mimics.Transwell assay was used to detect cell migration and invasion.Finally,we verified the targeting of PTP1B by miR-338-3p by the dual luciferase reporter gene.Results:In this study,we found that PTP1B protein level was obviously increased in gastric cancer tissues and that PTP1B promoted the migration and invasion of gastric cancer cell lines.Bioinformatics analysis identified that,miR-338-3p might potentially target PTP1B.In gastric cancer tissue,miR-338-3p was negatively correlated with PTP1B (r=-0.653,P=0.013).We demonstrated that miR-338-3p regulated PTP1B in gastric cancer cells and that it regulated the migration and invasion of gastric cancer cells.Conclusion:miR-338-3p can inhibit the migration and invasion of gastric cancer cells,which may be related to targeting PTP1B,which may provide new insights into novel molecular therapeutic targets for gastric cancer.

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东北地区重大慢病防控科技综合示范研究（编号：2018YFC1311600）
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