|Table of Contents|

The clinical diagnostic value of serum miR-122 and miR-570 combined detection in primary hepatic carcinoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 02
Page:
267-270
Research Field:
Publishing date:

Info

Title:
The clinical diagnostic value of serum miR-122 and miR-570 combined detection in primary hepatic carcinoma
Author(s):
YUAN Chong1; ZHAO Zheng2; LIAO Zijun2; ZHAO Bin2; ZHANG Yili2
1.Department of Clinical Laboratory,the First Affiliated Hospital of Air Force Medical University,Shaanxi Xi’an 710032,China;2.Shaanxi Provincial Cancer Hospital,Shaanxi Xi’an 710061，China.
Keywords:
primary hepatic carcinoma; miR-122; miR-570; miR-122 and miR-570 combined detection
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2021.02.018
Abstract:
Objective:To investigate the expression and the significance of serum miR-122 and miR-570 in the serum of primary hepatic carcinoma (PHC).Methods:The information of 30 patients with primary hepatocellular carcinoma treated in our hospital from February 2017 to January 2019 were selected as study group.Thirty cases of health examination in our hospital were selected as control group.Real-time fluorescence quantitative PCR was used to detect the expression of serum miR-122 and miR-570.The diagnostic efficacy of serum miR-122 and miR-570 was evaluated by receiver operating characteristic curve.Results:The expression levels of miR-122 and miR-570 in PHC patients were significantly lower than those in healthy controls.Differences were statistically significant (P<0.01).The expression level of miR-122 was negatively correlated with TNM staging,pathological grading and distant metastasis of PHC (P<0.05).There was no correlation between miR-570 expression level and TNM stage,pathological grade and distant metastasis degree in PHC.The AUC of miR-122 in PHC was 0.816.The sensitivity was 80.5%.The specificity was 90.1%.The AUC of miR-570 in PHC was 0.862.The sensitivity was 83.3%.The specificity was 90.0%.The AUC of combined serum miR-122 and miR-570 in PHC was 0.903.The sensitivity was 90.0%.The specificity was 83.6%.Conclusion:The serum miR-122 and miR-570 of PHC patients is lower than the control group.The expression level of serum miR-122 was negatively correlated with TNM stage,pathological grade and distant metastasis of PHC.The combined detection of serum miR-122 and miR-570 has high reference value for the diagnosis of PHC.

References:

［1］FERLAY J,COLOMBET M,SOERJOMATARAM I,et al.Estimating the global cancer incidence and mortality in 2018［J］.International Journal of Cancer,2019,144(8):1941-1953.
［2］RAJESHA RUPAIMOOLE,FRANK J SLACK.MicroRNA therapeutics:towards a new era for the management of cancer and other diseases［J］.Nat Rev Drug Discov,2017,12(3):203-222.
［3］VERDUCI L,STRANO S,YARDEN Y,et al.The circRNA-microRNA code:emerging implications for cancer diagnosis and treatment［J］.Molecular Oncology,2019,13(4):669-680.
［4］WU Q,LIU HO,LIU YD,et al.Decreased expression of hepatocyte nuclear factor 4α (Hnf4α)/microRNA-122 (miR-122) axis in hepatitis B virus-associated hepatocellular carcinoma enhances potential oncogenic GALNT10 protein activity［J］.The Journal of Biological Chemistry,2015,290(2):1170-1185.
［5］LIN YX,LIU S,SU L,et al.miR-570 inhibits proliferation,angiogenesis,and immune escape of hepatocellular carcinoma［J］.Cancer Biotherapy and Radiopharmaceuticals,2018,33(6):252-257.
［6］应倩,汪媛.肝癌流行现况和趋势分析［J］.中国肿瘤,2020,29(03):185-191. YING Q,WANG Y.Current situation and trend analysis of hepatocellular carcinoma［J］.Chinese Oncology,2020,29(03):185-191.
［7］XIE KL,ZHANG YG,LIU J,et al.MicroRNAs associated with Hbv infection and Hbv-related Hcc［J］.Theranostics,2014,12(4):1172-1196.
［8］TSAI WEI-CHIH,HSU SHENG-DA,HSU CHU-SUI,et al.MicroRNA-122 plays a critical role in liver homeostasis and hepatocarcinogenesis［J］.Journal of Investigative Medicine,2012,122(8):2884-2897.
［9］C COULOUARN,VM FACTOR,JB ANDERSEN,et al.Loss of miR-122 expression in liver cancer correlates with suppression of the hepatic phenotype and gain of metastatic properties［J］.Oncogene，2009,28(40):3526-3536.
［10］WEI-CHIH TSAI,PAUL WEI-CHE HSU,TSUNG-CHING LAI,et al.MicroRNA-122,a tumor suppressor microRNA that regulates intrahepatic metastasis of hepatocellular carcinoma［J］.Hepatology,2009,6(5):1571-1582.
［11］张楚红,胡甜,阳学风,等.循环microRNA-122a和microRNA-6086在肝癌诊断及预后中的作用［J］.湖南师范大学学报(医学版),2017,14(02):12-16. ZHANG CH,HU T,YANG XF,et al.The role of circulating microRNA-122a and microRNA-6086 in the diagnosis and prognosis of liver cancer ［J］.Journal of Hunan normal University (Medical Edition),2017,14(02):12-16.
［12］GUO W,TAN W,LIU S,et al.MiR-570 inhibited the cell proliferation and invasion through directly targeting B7-h1 in hepatocellular carcinoma［J］.Tumour Biology,2015,36(11):9049-9057.
［13］亓艳,吴天歌,王照艳.甲胎蛋白及甲胎蛋白异质体L3对原发性肝细胞癌的诊断价值［J］.癌症进展,2020,18(17):1774-1777. RUAN Y,WU TG,WANG ZY.The diagnostic value of alpha-fetoprotein and alpha-fetoprotein heteromer l3 in primary hepatocellular carcinoma ［J］.Cancer Progression,2020,18(17):1774-1777.
［14］胡广梅,钟日辉,黄利思,等.甲胎蛋白、α-L-岩藻糖苷酶、糖链抗原125血清检测在原发性肝癌筛查及鉴别诊断中的价值［J］.中国卫生检验杂志,2020,30(17):2052-2055. HU GM,ZHONG RH,HUANG LS,et al.The value of 125 serum detection of alpha fetoprotein,α-l- fucosidase and sugar chain antigen in screening and differential diagnosis of primary liver cancer ［J］.Chinese Journal of Health Inspection,2020,30(17):2052-2055.
［15］许丽娜,黄东凤,李峰,等.血清miRNA-122和miRNA-221在原发性肝癌患者的表达及其诊断价值［J］.江苏医药,2015,41(11):1285-1288. XU LN,HUANG DF,LI F,et al.The expression and its diagnostic value of serum miRNA-122 and miRNA-221 in patients with primary liver cancer ［J］.Jiangsu Medicine,2015,41(11):1285-1288.
［16］汪晓华,陈晓军.肝癌患者外周血外泌体miR-25和miR-141表达水平及联合检测诊断效能研究［J］.中国卫生检验杂志,2020,30(4):472-474. WANG XH,CHEN XJ.Study on the miR-25 and miR-141 expression of peripheral blood exocrine in liver cancer patients and the diagnostic efficacy of combined detection［J］.Chinese Journal of Health Inspection,2020,30(4):472-474.
［17］陈泽衍,陈晓静,郑文斌,等.miR-146a、miR-375联合AFP检测在诊断原发性肝癌中的应用［J］.中国医学创新,2019,16(36):132-135.CHEN ZY,CHEN XJ,ZHENG WB,et al.miR-146a,miR-375 combined AFP detection in diagnosis of primary hepatocellular carcinoma［J］.Chinese Medical Innovation,2019,16(36):132-135.

Memo

Memo:
-
Last Update: 1900-01-01