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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2021 02

Page:

232-238

Research Field:

Publishing date:

Info

Title:

HIF-1¦Á promotes migration and invasion of breast cancer cells by down-regulating TP53INP1

Author(s):

WANG Yi¹; ZHANG Yusong¹; LUO Wenjun²; LIU Shuang³; XIA Qingxin¹

1.Cancer Hospital Affiliated Zhengzhou University£¬Henan Zhengzhou 450008,China;2.General Hospital of Tianjin Medical University,Tianjin 300052,China;3.Basic Medical School,Tianjin Medical University,Tianjin 300070,China.

Keywords:

breast cancer; HIF-1¦Á; TP53INP1; migration and invasion

PACS:

R737.9

DOI:

10.3969/j.issn.1672-4992.2021.02.011

Abstract:

Objective:To investigate the effect that HIF-1¦Á on breast cancer cells migration and invasion by the regulation of TP53INP1.Methods:Immunohistochemistry staining showed the expression of TP53INP1 and HIF-1¦Á in 60 breast cancer patients tissues and the clinicopathological data were analyzed.Wound-healing assay,invasion and migration assay confirmed the influence of breast cancer migration and invasion on interaction between TP53INP1 and HIF-1¦Á.The expression of MMP2£¬VEGF related protein markers were tested by Western blot in cotransfected shHIF-1 and TP53INP1 or shTP53INP1 breast cancer cells.Results:The expression of HIF-1¦Á was high and TP53INP1 was low in breast cancer tissues and there is association between HIF-1¦Á and TP53INP1.Furthermore,HIF-1¦Á and TP53INP1 expression were correlated with lymph node metastasis£¨P£¼0.05£©.Downregulation of HIF-1¦Á could inhibit the ability of breast cancer cells migration and invasion,however,cotransfected shHIF-1¦Á and shTP53INP1 MCF-7 cells reversed the ability of breast cancer cells migration and invasion£¨P£¼0.05£©.Western blot results showed that the expression of MMP2 was high in cotransfected shHIF-1¦Á and TP53INP1 MDA-MB-231 cells,the expression of VEGF and MMP2 were high in cotransfected shHIF-1¦Á and shTP53INP1 MCF-7 cells£¨P£¼0.05£©.Conclusion:HIF-1¦Á promote ability of breast cancer cells migration and invasion by the downregulation of TP53INP1.

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