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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2021 02

Page:

225-232

Research Field:

Publishing date:

Info

Title:

Expression and clinical significance of calcium channel protein ¦Á2¦Ä1 and miR-107 in laryngeal carcinoma

Author(s):

HUANG Chaoping¹; WANG Yi¹; HUANG Shi¹; LIU Liangfa²

1.Department of Otolaryngology Head and Neck Surgery,the First Affiliated Hospital of Chengdu Medical College,Sichuan Chengdu 610500,China;2.Department of Otolaryngology Head and Neck Surgery,Beijing Friendship Hospital,Capital Medical University,Beijing 100000,China.

Keywords:

laryngeal cancer; ¦Á2¦Ä1; microRNA-107; CACNA2D1

PACS:

R739.65

DOI:

10.3969/j.issn.1672-4992.2021.02.010

Abstract:

Objective:To detect the expression of calcitonin ¦Á2¦Ä1 and miR-107 in laryngeal squamous cell carcinoma,explore their possible role in the pathogenesis of laryngeal cancer.Methods:We collected 40 cases of laryngeal cancer patients and adjacent normal tissues,and detected the expression of ¦Á2¦Ä1 and its coding genes CACNA2D1 and miR-107 in two tissues,and analyze whether their expression is related to the clinical characteristics and prognosis of patients.Bioinformatics technology was used to find potential binding sites of CACNA2D1 and miR-107.The dual-luciferase reporter gene was used for verification.Cell migration and proliferation experiments were performed after overexpression and knockdown of miR-107 using laryngeal cancer cell TU212.Results:The results of immunofluorescence and Western blot analysis showed that the expression of ¦Á2¦Ä1 was higher in laryngeal carcinoma than in adjacent normal tissues (P£¼0.05).And the RT-qPCR analysis showed that the expression level of miR-107 was significantly decreased in laryngeal carcinoma tissues compared with adjacent normal tissues (P£¼0.05).The low expression of miR-107 was associated with lymph node metastasis,tumor differentiation and primary site in patients with laryngeal cancer (P£¼0.05),but not with age and tumor TNM stage (P£¾0.05).The high expression of CACNA2D1 was closely related to lymph node metastasis,tumor differentiation and TNM staging in patients with laryngeal cancer (P£¼0.05),but not related to the age of LSCC patients and the primary site of tumor (P£¾0.05).The expression of CACNA2D1 was correlated with patients' relapse and three-year survival rate (P£¼0.05),while miR-107 was not correlated with it (P£¾0.05).Dual-luciferase reporter gene experiments confirmed that miR-107 can bind to two sites on the 3¡ä-UTR end of CACNA2D1.Overexpression of miR-107 in TU212 cells inhibited cell migration and proliferation,and knockdown enhanced migration and proliferation of laryngeal cancer cells (P£¼0.05).Conclusion:Calcium channel protein ¦Á2¦Ä1 plays a cancer-promoting role in patients with laryngeal squamous cell carcinoma.miR-107 can directly target CACNA2D1 to regulate the migration and proliferation of laryngeal cancer cells.

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