|Table of Contents|

Gold nanoclusters assisted delivery of PD-L1 siRNA for anti-tumor activity in oral squamous cell carcinoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 02
Page:
206-210
Research Field:
Publishing date:

Info

Title:
Gold nanoclusters assisted delivery of PD-L1 siRNA for anti-tumor activity in oral squamous cell carcinoma
Author(s):
CHEN Chuangmao; SONG Jingqin; CHEN Zhijun; CHEN Wen
Maxillofacial Surgery,the Second Affiliated Hospital of Hainan Medical College,Hainan Haikou 570311,China.
Keywords:
oral squamous cell carcinoma; gold nanoclusters; siRNA; PD-L1
PACS:
R739.8
DOI:
10.3969/j.issn.1672-4992.2021.02.006
Abstract:
Objective:To investigate whether PD-L1 siRNA delivered by gold nanoclusters can enhance the anti-tumor effect of lymphocytes on oral squamous cell carcinoma HSC-4 cells.Methods:The gold nanoclusters were reacted in one step,and the gold nanoclusters-siRNA complex was formed with PD-L1 siRNA.The free siRNA and gold nanoclusters siRNA complex were incubated with HSC-4 cells respectively.The knockdown of PD-L1 was detected by qPCR and Western blot.The secretion of cytokine IL-2 of T cells was detected by ELISA,and the anti-tumor activity was detected by establishing mouse tumor model.Results:Gold nanoclusters-siRNA complex was successfully produced and could effectively knock down the expression of PD-L1 in HSC-4 cells.After T cells were co-incubated with tumor cells,gold nanoclusters-siRNA could significantly increase the secretion of cytokine IL-2 compared with free siRNA.In vivo experiments showed that,compared with free siRNA,gold nanoclusters-siRNA can better target tumor tissue and enhance anti-tumor ability of T cells in HSC-4 tumors.Conclusion:The PD-L1 siRNA system delivered by gold nanoclusters can enhance the anti-tumor effect of PD-L1 siRNA in tumors and reduce the expression of PD-L1 in oral squamous cell carcinoma,thus enhancing the anti-tumor activity of T cells against oral squamous cell carcinoma.This suggests that this gold nanoclusters-PD-L1-siRNA complex may be a promising strategy for the treatment of oral squamous cell carcinoma.

References:

［1］SHIA BC,QIN L,LIN KC,et al.Outcomes for elderly patients aged 70 to 80 years or older with locally advanced oral cavity squamous cell carcinoma:A propensity score-matched,nationwide,oldest old patient-based cohort study［J］.Cancers (Basel),2020,12(2):E258.
［2］DASKALOPOULOS AG,AVGOUSTIDIS D,CHAISUPARAT R,et al.Assessment of TLR4 and TLR9 signaling and correlation with human papillomavirus status and histopathologic parameters in oral tongue squamous cell carcinoma［J］.Oral Surg Oral Med Oral Pathol Oral Radiol,2020,129(5):493-513.
［3］ACHARYA S,PRABHU P,PATIL V,et al.Tumor necrosis factor-like weak inducer of apoptosis expression in healthy oral mucosa,oral dysplasia and oral squamous cell carcinoma［J］.J Oral Maxillofac Pathol,2019,23:369-377.
［4］LEE YS,JOHNSON DE,GRANDIS JR.An update:Emerging drugs to treat squamous cell carcinomas of the head and neck［J］.Expert Opin Emerg Drugs,2018,23(4):283-299.
［5］HAZEKAWA M,NISHINAKAGAWA T,KAWAKUBO-YASUKOCHI T,et al.Glypican-3 gene silencing for ovarian cancer using siRNA-PLGA hybrid micelles in a murine peritoneal dissemination model［J］.J Pharmacol Sci,2019,139:231-239.
［6］PENG S,LU Y,LI P,et al.The short interference RNA (siRNA) targeting NMUR2 relieves nociception in a bone cancer pain model of rat through PKC-ERK and PI3K-AKT pathways［J］.Biochem Biophys Res Commun,2019,512:616-622.
［7］SHABANI S,MAHJOUBI F,MOOSAVI MA.A siRNA-based method for efficient silencing of PYROXD1 gene expression in the colon cancer cell line HCT116［J］.J Cell Biochem,2019,120:19310-19317.
［8］GUAN ZJ,HU F,LI JJ,et al.Isomerization in alkynyl-protected gold nanoclusters［J］.J Am Chem Soc,2020,142:2995-3001.
［9］HOBO W,MAAS F,ADISTY N,et al.siRNA silencing of PD-L1 and PD-L2 on dendritic cells augments expansion and function of minor histocompatibility antigen-specific CD8+ T cells［J］.Blood,2010,116:4501-4511.
［10］HAYES RB,AHN J,FAN X,et al.Association of oral microbiome with risk for incident head and neck squamous cell cancer［J］.JAMA Oncol,2018,4(3):358-365.
［11］MALASPINA TS,GASPAROTO TH,COSTA MR,et al.Enhanced programmed death 1 (PD-1) and PD-1 ligand (PD-L1) expression in patients with actinic cheilitis and oral squamous cell carcinoma［J］.Cancer Immunol Immunother,2011,60:965-974.
［12］TROEITZSCH M,WOODLCK T,PIANKA A,et al.Is there evidence for the presence and relevance of the PD-1/PD-L1 pathway in oral squamous cell carcinoma?Hints from an immunohistochemical study［J］.J Oral Maxillofac Surg,2017,75:969-977.
［13］SHARMA A,KUMAR P,AMBASTA RK.Cancer fighting SiRNA-RRM2 loaded nanorobots［J］.Pharm Nanotechnol,2020,8(2):79-90.
［14］MAYSINGER D,GRAN ER,BERTORELLE F,et al.Gold nanoclusters elicit homeostatic perturbations in glioblastoma cells and adaptive changes of lysosomes［J］.Theranostics,2020,10:1633-1648.
［15］PORRET E,LE GUEVEL X,COLL JL.Gold nanoclusters for biomedical applications:Toward in vivo studies［J］.J Mater Chem B,2020,8(11):2216-2232.
［16］LIU Y,WU L,TONG R,et al.PD-1/PD-L1 inhibitors in cervical cancer［J］.Front Pharmacol,2019,10:65.
［17］SHEN K,CUI J,WEI Y,et al.Effectiveness and safety of PD-1/PD-L1 or CTLA4 inhibitors combined with chemotherapy as a first-line treatment for lung cancer:A meta-analysis［J］.J Thorac Dis,2018,10:6636-6652.

Memo

Memo:
海南省卫生健康行业科研项目（编号：19A200007）
Last Update: 1900-01-01