|Table of Contents|

siRNA-mediated silencing of eIF3b gene inhibits the invasion and migration of breast cancer cells MCF-7

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 02
Page:
201-205
Research Field:
Publishing date:

Info

Title:
siRNA-mediated silencing of eIF3b gene inhibits the invasion and migration of breast cancer cells MCF-7
Author(s):
LI Yanhui1; FANG Mao2; XIE Minru1; XIA Minghan3
1.Department of Pathology,Shijie Hospital,Guangdong Dongguan 523290,China;2.Pathology Department,Guangzhou Medical University，Guangdong Guangzhou 511483,China;3.Department of Pathology，the First Hospital of Jinan University,Guangdong Guangzhou 510000,China.
Keywords:
eIF3b; breast cancer; migration; invasion; RNA interference
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2021.02.005
Abstract:
Objective:To investigate the influence of RNA interference induced silencing of eIF3b gene on migration and invasion potential in breast cancer cells MCF-7.Methods:Human breast cancer cells MCF-7 were divided into control group(I group)，scramble group(II group) and si eIF3b group(III group).siRNA sequence targeting eIF3b gene was designed and synthe sized．The siRNA was transfected into breast cancer cells MCF-7 by lipofectamineTM 2000 mediation．The alteration of eIF3b mＲNA and protein expression was detected by qＲT-PCＲ and Western blotting．Invasion potential was evaluated by Transwell invasion assay．Cell migrating ability was detected by Transwell migration assay．Results:eIF3b protein expression of III group （0.45±0.06） was lower than that of I group （1.09±0.19） and II group（1.05±0.17）(P＜0.05).There was no difference between I group and II group(P＞0.05).eIF3b mＲNA expression of III group (0.54±0.08) was lower than that of I group (1.12±0.16) and II group(1.08±0.18)(P＜0.05).There was no difference between I group and II group(P＞0.05).In cell migration assay,the number of cells in III group was less than that in I group and II group(P＜0.05).In invasion potential test,the number of cells in III group was less than that in I group and II group(P＜0.05).Conclusion:The siRNA targeting eIF3b gene can restrain the migration and invasion of breast cancer cell.

References:

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2019年东莞市社会科技发展（重点）项目(编号：201950715011207)
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