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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2021 02

Page:

196-201

Research Field:

Publishing date:

Info

Title:

Silencing RAI14 expression inhibits gastric cancer cell proliferation,invasion,migration and angiogenesis

Author(s):

LIU Chengye; HE Xinyang

The Affiliated Provincial Hospital of Anhui Medical University,Anhui Hefei 230001,China.

Keywords:

gastric cancer; RAI14; proliferation; invasion; migration; angiogenesis

PACS:

R735.2

DOI:

10.3969/j.issn.1672-4992.2021.02.004

Abstract:

Objective:To investigate the effect of short hairpin RNA (shRNA) targeted silencing of Retinoic Acid-Induced Protein 14 (RAI14) expression on the proliferation,invasion,migration and angiogenesis of gastric cancer cells.Methods:Firstly,the successfully constructed shRNA vector fragment (silenced group) or nonsense shRNA fragment (control group) targeting silenced RAI14 expression was randomly transfected into gastric cancer cell line MKN-45.Then real-time fluorescent quantitative PCR (qRT-PCR) was used to detect the expression of RAI14 mRNA.Western blotting was used to detect the expression of RAI14 protein and p-AKT.Clone formation and cell count kit 8 (CCK-8) was used to detect the proliferation.Transwell test and scratch test were used to detect the invasion and migration ability of cells respectively,and the effect of RAI14 on angiogenesis of gastric cancer was analyzed in vitro.Results:qRT-PCR and Western blotting showed that the levels of RAI14 mRNA and protein in the silence group were significantly lower than those in the control group (P£¼0.05).The proliferation,invasion,migration and angiogenesis of gastric cancer cells in the silence group were significantly lower than those in the control group (P£¼0.05).The relative expression of p-AKT protein in the silence group was 0.44¡À0.01,lower than that in the control group (1.00¡À0.08),the difference was statistically significant (P£¼0.05).Conclusion:Silencing the expression of RAI14 can inhibit the proliferation,invasion,migration and angiogenesis of gastric cancer cells,which may be related to the down-regulation of p-AKT protein expression.

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