|Table of Contents|

Changes of the expression of CD45+,CD68+,CD163+ and CD11b+ immune cells in mouse lung tissue after surgical resection of tumor-bearing lymph node

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 02
Page:
181-184
Research Field:
Publishing date:

Info

Title:
Changes of the expression of CD45+,CD68+,CD163+ and CD11b+ immune cells in mouse lung tissue after surgical resection of tumor-bearing lymph node
Author(s):
JIANG Yourong1; JIA Limin2; HE Zhijin2; ZHENG Jinhua2
1.Department of Oral Histopathology,the Affiliated Stomatological Hospital of Harbin Medical University,Heilongjiang Harbin 150001,China;2.Department of Anatomy,Harbin Medical University,Heilongjiang Harbin 150081,China.
Keywords:
surgery; tumor-bearing lymph node; lung; immune cells; microenvironment; mouse
PACS:
R730.56
DOI:
10.3969/j.issn.1672-4992.2021.02.001
Abstract:
Objective:To investigate the effect of surgical resection of tumor-bearing lymph node on the expression of immune cells in mouse lung tissue.Methods:Mouse B16F10 melanoma cells were inoculated into mouse subiliac lymph node (SiLN).15 days later,tumor-bearing lymph node were surgically removed.All mice were divided into the surgical removal of SiLN group and the non-surgical removal group (control group).We used HE staining and Elastic-Masson staining to observe changes of general morphological structure and collagen fibers in mouse lung tissue,and applied immunohistochemistry to observe changes of CD45+,CD68+,CD163+,CD11b+ immune cells in mouse lung tissue.Results:Compared with the control group,pulmonary inflammatory cells infiltration in the surgical removal of lymph node group was significantly increased.The stromal collagen fibers around the blood vessels in lung tissues were sparse.The expression of CD45+ total inflammatory cells,CD68+ macrophages,CD163+ M2 macrophages and CD11b+ myeloid-derived suppressor cells (MDSCs) in mouse lung tissue were significantly increased(P＜0.05).Conclusion:Surgical removal of tumor-bearing lymph node can promote the expression of CD163+ M2 macrophages and CD11b+ MDSC in mouse lung tissue,which is helpful to the formation of distant organ microenvironment that supports tumor cell colonization.

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Memo:
黑龙江省自然科学基金（编号：H2017015）
Last Update: 1900-01-01