|Table of Contents|

The mechanisms of erythropoietin-producing hepatocellular A2 protein participating in the invasion of hepatocellular carcinoma cells via regulating the expression of growth factor receptor bound protein 2

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 01
Page:
32-37
Research Field:
Publishing date:

Info

Title:
The mechanisms of erythropoietin-producing hepatocellular A2 protein participating in the invasion of hepatocellular carcinoma cells via regulating the expression of growth factor receptor bound protein 2
Author(s):
WANG Yanan; MA Qingjiu; XU Jianqing; MAI Saihu; HUANG Weihua; YANG Xijia; WANG Qi; FAN Weiwei; WANG Shangyu; ZHOU Liang
Department of General Surgery，Xi'an High-tech Hospital Affiliated to Xi'an Medical College，Shaanxi Xi'an 710075，China.
Keywords:
liver cancer; erythropoietin-producing hepatocellular A2; growth factor receptor bound protein 2; invasion
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2021.01.007
Abstract:
Objective：To inquire into the expression and clinical significance of EphA2 protein in the invasion of hepatocellular carcinoma cells, and the possible mechanism of regulating hepatocellular carcinoma cells.Methods:We cultured hepatic cell HL-7702 ，HCC cell lines MHCC97H and Hep3B.The expression of EphA2 and Grb2 in the MHCC97H and Hep3B was suppressed by siRNA interference, and then were divided into three groups：Untreated group,the control group and the siRNA intervention group.The protein expression of EphA2 and Grb2 was explored by Western blot, the invasion ability of MHCC97H and Hep3B was explored by Transwell chamber.Results:The numbers of HL-7702,Hep3B and MHCC97H cells penetrated the watrigel were（31±3）/10 HPF,（111±4）/10 HPF,（401±3）/10 HPF, respectively.There was significant difference in the number between hepatic cell and HCC cell lines（P<0.05）.There was significant difference in the protein expression of EphA2 and Grb2 between hepatic cell and HCC cell lines（P<0.05）.Suppress the expression of EphA2, the numbers of Hep3B and MHCC97H cells penetrated the Watrigel in the untreated group ,the control group and the siRNA intervention group were（111±4）/10 HPF,（108±5）/10 HPF,（53±3）/10 HPF，and （405±5）/10 HPF,（399±4）/10 HPF,（155±7）/10 HPF.There was significant difference in the number between the control group and the siRNA intervention group（P<0.001）.There was significant difference in the protein expression of EphA2 in the MHCC97H and Hep3B cells between the control group and the siRNA intervention group（P<0.05）.Suppress the expression of Grb2, the numbers of Hep3B and MHCC97H cells penetrated the watrigel in the untreated group,the control group and the siRNA intervention group were（109±3）/10 HPF,（107±4）/10 HPF,（56±4）/10 HPF and（403±4）/10 HPF,（402±4）/10 HPF,（163±5）/10 HPF.There was significant difference in the number between the control group and the siRNA intervention group（P<0.001）.There was significant difference in the protein expression of Grb2 in the MHCC97H and Hep3B cells between the control group and the siRNA intervention group（P<0.05）.Suppress the expression of EphA2, there was significant difference in the protein expression of Grb2 in the MHCC97H and Hep3B cells between the control group and the siRNA intervention group（P<0.05）.Conclusion:EphA2 may participate in the invasion of HCC cells by regulating the expression of Grb2 protein.EphA2 may be a new target for the treatment of HCC.

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Memo

Memo:
陕西省卫生健康科研基金项目（编号：2018D008）
Last Update: 2020-11-30