|Table of Contents|

miR-145 inhibits the invasion of non-small cell lung cancer cells by targeting inhibition of SMAD3 expression

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 01
Page:
26-31
Research Field:
Publishing date:

Info

Title:
miR-145 inhibits the invasion of non-small cell lung cancer cells by targeting inhibition of SMAD3 expression
Author(s):
HE Gaoyan; LUO Xiaobin; ZHAO Yong; LUO Li
Department of Respiratory and Critical Medicine，Suining City Central Hospital,Sichuan Suining 629000，China.
Keywords:
miR-145; non-small cell lung cancer; SMAD3; invasion
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2021.01.006
Abstract:
Objective：To investigate the effect of miR-145 targeting the regulation of SMAD3 expression on the invasive ability of non-small cell lung cancer cells.Methods：The expression levels of miR-145 and SMAD3 in 30 non-small cell lung cancer tissues and adjacent tissues were detected by qRT-PCR and their correlations were analyzed.The target gene prediction site was used to predict the potential target gene SMAD3 of miR-145，which was verified by the dual luciferase reporter gene assay.The transfected cells of miR-145 mimics，miR-145 inhibitor，siRNA SMAD3 and related controls were transfected into non-small cell lung cancer A549 cells by cell transfection experiments.Transwell assay was used to detect the invasive ability of A549 cells after transfection.The effect of miR-145 on the expression of SMAD3 protein was up-regulated or down-regulated in A549 cells by Western blot.Results：The results of qRT-PCR showed that miR-145 was down-regulated and SMAD3 was highly expressed in non-small cell lung cancer tissues，and the expression levels of both were negatively correlated.Target gene prediction and validation experiments showed that miR-145 can specifically bind to the 3'-UTR of SMAD3，which was a target gene of miR-145.Western blot analysis showed that transfection of miR-145 mimics significantly decreased the expression of SMAD3 protein(P<0.001)，and transfection of miR-145 inhibitor significantly increased the expression of SMAD3 protein(P<0.01).Transwell in vitro invasion assay showed that the transfected miR-145 mimic group significantly reduced the invasive ability of A549 cells compared with the control group(P<0.001)，and the transfected miR-145 inhibitor group significantly increased the invasive ability of A549 cells(P<0.05).Compared with the control group，the invasive ability of knockdown of SMAD3 expressing A549 cells was weakened(P<0.001).The invasive ability of co-transfected siRNA SMAD3 and miR-145 mimics A549 cells was weaker than that of knockdown of SMAD3 alone(P<0.05).There was no significant difference in the invasive ability of co-transfected siRNA SMAD3 and miR-145 inhibitor A549 cells(P>0.05).Conclusion：miR-145 is lowly expressed in non-small cell lung cancer tissues，and miR-145 exerts an inhibitory effect on the invasive ability of non-small cell lung cancer cells by targeting inhibition of SMAD3 expression，providing a new target for clinical comprehensive treatment.

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Last Update: 2020-11-30