|Table of Contents|

Effects of n-3 polyunsaturated fatty acids on proliferation and apoptosis of esophageal carcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 01
Page:
22-26
Research Field:
Publishing date:

Info

Title:
Effects of n-3 polyunsaturated fatty acids on proliferation and apoptosis of esophageal carcinoma cells
Author(s):
SHEN Dandan; XU Huixian; DENG Guosun
Department of Gastroenterology，Chongming Branch of Xinhua Hospital Affiliated to Shanghai Jiao Tong University，Shanghai 202150，China.
Keywords:
n-3 polyunsaturated fatty acids; esophageal cancer; cell proliferation; cell apoptosis
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2021.01.005
Abstract:
Objective：To investigate the role of DHA and EPA，two active components of n-3 polyunsaturated fatty acids，in the proliferation and apoptosis of esophageal cancer cells.Methods：CCK-8 assay was used to determine the effects of DHA and EPA at different concentrations and time points(24 h，48 h，72 h after culture) on the proliferation of esophageal cancer cells.Western-blot method was used to detect the effects of DHA and EPA on apoptosis-related proteins.Fluorescence staining and flow cytometry were used to detect the effects of DHA and EPA on the apoptosis of esophageal cancer cells.Results：Compared with the control group，both DHA and EPA could inhibit the proliferation of esophageal cancer cells in a concentration dependent but not time-dependent manner.Western-blot showed that DHA and EPA could activate Caspase-3 and Bax and inhibit the anti-apoptotic protein Bcl-2.After 24 hours of DHA and EPA treatment，fluorescence staining showed typical apoptotic morphological changes in some esophageal cancer cells.Flow cytometry showed that DHA and EPA could induce apoptosis of esophageal cancer cells，which was proportional to the concentration，compared with the control group.Conclusion：n-3 polyunsaturated fatty acids can inhibit the proliferation and induce the apoptosis of esophageal cancer cells.Regulating the balance between Caspase-3 activity and the Bcl-2 family of pro-apoptotic proteins/anti-apoptotic proteins can play its role in inhibiting the proliferation of esophageal cancer cells and inducing the apoptosis of esophageal cancer cells.

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Last Update: 2020-11-30