|Table of Contents|

Silence MALAT1 increases miR-1 expression and increases sensitivity to 5-FU in gallbladder carcinoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 03
Page:
391-396
Research Field:
Publishing date:

Info

Title:
Silence MALAT1 increases miR-1 expression and increases sensitivity to 5-FU in gallbladder carcinoma
Author(s):
Zhang Zhibiao1; Yu Wei2; Ma Da2; Liu Lijun2
1.Emergency Department；2.General Surgery Department，Hanchuan People's Hospital，Hubei Hanchuan 431600，China.
Keywords:
gallbladder carcinoma; MALAT1; miR-1; apoptosis; 5-FU
PACS:
R735.8
DOI:
10.3969/j.issn.1672-4992.2020.03.009
Abstract:
Objective：To clarify the effect of MALAT1 on the sensitivity of gallbladder carcinoma (GBC) cells to 5-FU through regulation of miR-1.Methods：qRT-PCR was used to detect the expression of MALAT1 and miR-1 in GBC cells GBC-SD，NOZ and SGC-996.MTT and flow cytometry were used to detect the proliferation and apoptosis of normal and 5-FU-treated GBC cells,and dual fluorescein reporter gene assay was used to detect the targeting relationship between MALAT1 and miR-1.Results：qRT-PCR results showed that compared with human normal gallbladder epithelial cells H69，MALAT1 was up-regulated in GBC cells GBC-SD，NOZ and SGC-996.Dual fluorescein reporter gene assay results showed that MALAT1 could target and regulate the expression of miR-1，and negatively regulate the expression of miR-1.MTT and flow cytometry showed that knocking down MALAT1 inhibited the proliferation and induced apoptosis of GBC cells，increased the sensitivity of GBC cells to 5-FU，and over-expression of miR-1 was consistent with knocking down MALAT1.Silencing MALAT1 partially reversed the effects of knocking down miR-1 inhibitor on the viability，apoptosis and 5-FU sensitivity of GBC cells.Conclusion：Silencing MALAT1 increased the expression of miR-1 and increased the sensitivity of GBC cells to 5-FU.

References:

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