|Table of Contents|

Therapeutic effects and mechanistic regulation of H19 controlling of estrogen induction of breast cancer stem-like cells' renewal

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 03
Page:
364-369
Research Field:
Publishing date:

Info

Title:
Therapeutic effects and mechanistic regulation of H19 controlling of estrogen induction of breast cancer stem-like cells' renewal
Author(s):
Zhang Chunlei1; Wang Meng2; Wu Jie2; Li Xiang2; Du Ning2; Ren Hong2; Huang Guanglin1
1.Department of General Surgery，Yulin Xingyuan Hospital，Shaanxi Yulin 719000，China；2.the Second Department of Thoracic Surgery，the First Affiliated Hospital of Xi'an Jiaotong University，Shaanxi Xi'an 710061，China.
Keywords:
breast cancer stem-like cells; lncRNA; H19; Let-7c; Wnt pathway
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2020.03.003
Abstract:
Objective：To explore the role and mechanism of long non-coding RNA H19 in controlling breast cancer stem-like cells (BrCSC) division.Methods：Sample data of H19 pre-clinical study were analyzed by TCGA database.The self-renewal ability was assessed by spheres formation.The mechanistic procedures were tested by RT-PCR，Western blot and Luciferase assay.Results：H19 activated the estrogen-maintained Wnt pathway activity in BrCSC by inhibiting the function of Let-7c.The activation of Wnt pathway further stimulated the increase of H19，reduced the bioavailability of Let-7c and formd a feedback loop.Similarly，restoring Let-7c expression would weaken the estrogen-dependent Wnt pathway，thereby inhibiting H19 and symmetrical division of BrCSC.Conclusion：H19 regulates Wnt pathway activity by binding and depending on Let-7c，and forms H19/Let-7/Wnt feedback loop，which plays a key role in the regulation of BrCSC's self-renewal ability.

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Memo

Memo:
National Natural Science Foundation of China(No.81602597）；国家自然科学基金（编号：81602597）；陕西省自然科学基金（编号：2018JM7017）
Last Update: 1900-01-01