|Table of Contents|

Study on the correlation between miR-147 and biological activity of U87 cells in glioma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 03
Page:
360-363
Research Field:
Publishing date:

Info

Title:
Study on the correlation between miR-147 and biological activity of U87 cells in glioma cells
Author(s):
Xu Xiaobin1Wu Weinan1Ma Ning2Lian Shizhong2
1.The First Clinical Medical College,Shanxi Medical University,Shanxi Taiyuan 030001,China;2.Department of Neurosurgery,the First Hospital of Shanxi Medical University,Shanxi Taiyuan 030001,China.
Keywords:
miR-147gliomaproliferationapoptosisinvasion
PACS:
R739.41
DOI:
10.3969/j.issn.1672-4992.2020.03.002
Abstract:
Objective:To compare the difference of miR-147 expression between glioma tissues and adjacent tissues,and to further analyze the correlation between miR-147 and biological activity of glioma cells.Methods:The expression of miR-147 in 65 glioma tissues and adjacent tissues was detected by real-time fluorescence quantitative PCR,and the glioma cells were transfected into miR-147 mimics group and NC group by Lipofectamine 2000 method,and the transfection efficiency of the two groups was detected.Furthermore,CCK-8 assay,flow cytometry test and Transwell assay were used to compare the cell biological activity between the two groups.Results:The expression level of miR-147 in glioma tissue was significantly lower than that in paratumor tissue,and compared with NC group,the proliferation and invasion ability of miR-147 mimics group were decreased,but the apoptosis rate was increased.Conclusion:There is a low expression of miR-147 in glioma tissues.Overexpression of miR-147 can inhibit the proliferation and invasion of glioma cells and induce apoptosis of glioma cells,which plays an anti-cancer role.

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