|Table of Contents|

The mechanism of Ku70 acetylation modification mediating apoptosis of colon cancer cells induced by trichostatin A (TSA)

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 03
Page:
355-360
Research Field:
Publishing date:

Info

Title:
The mechanism of Ku70 acetylation modification mediating apoptosis of colon cancer cells induced by trichostatin A (TSA)
Author(s):
Meng Jin12Liu Xinli3Che Ling1Chen Ming1Wu Man1Ma Chenke1Zhao Guanren1
1.Department of Pharmacy,the Eighth Medical Center of Chinese PLA General Hospital,Beijing 100091,China;2.Department of Oncology,Tangdu Hospital,Air Force Medical University,Shaanxi Xi'an 710038,China;3.College of Life,Northwestern Polytechnical University,Shaanxi Xi'an 710072,China.
Keywords:
colon cancertrichostatin A (TSA)acetylationapoptosis
PACS:
R735.3+5
DOI:
10.3969/j.issn.1672-4992.2020.03.001
Abstract:
Objective:To investigate the effect and mechanism of Ku70 acetylation modification on the apoptosis of colon cancer cells induced by trichostatin A (TSA).Methods:Colon cancer HCT116 cells and SW620 cells were selected and cultured in vitro and treated with concentration gradient TSA.MTT assay was used to detect the IC50 and cell viability treated with TSA.Western blot and immunofluorescence staining assays were used to detect the effects of TSA on the protein level and intracellular distribution of Ku70 and acetyl-Ku70.The flow cytometry detected the effect of TSA on apoptosis of colon cancer HCT116 cells and SW620 cells.The effects of TSA on the expression of apoptosis-related proteins,including Caspase-3,Bax and Bcl-2 in colon cancer HCT116 cells and SW620 cells were detected by Western blot.Results:MTT assay results showed that TSA could inhibit the activity and growth of colon cancer HCT116 and SW620 cells in a concentration-dependent manner with an IC50 value of 1.023 μmol/L and 1.076 μmol/L (P<0.05).Western blot and immunofluorescence staining results showed that TSA could significantly up-regulate the protein level of acetyl-Ku70 in colon cancer HCT116 and SW620 cells and promote its nuclear transduction (P<0.05).Flow cytometry results showed that TSA could significantly promote colon cancer HCT116 and SW620 cells apoptosis (P<0.05).In addition,Western blot results showed that TSA could significantly up-regulate the apoptosis protein Caspase-3 and Bax in colon cancer HCT116 and SW620 cells,and down-regulate the expression of anti-apoptosis protein Bcl-2 (P<0.05).Conclusion:In the colon cancer HCT116 and SW620 cells,TSA may play a role in targeting the Ku70 post-translational modification regulation by mediating the acetylation of the proto-oncogene Ku70 and promoting its nuclear transduction to promote its apoptosis,and providing a new strategy and theoretical basis for the anti-treatment of colon cancer cells.

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Memo

Memo:
陕西省自然科学基础研究计划项目(编号:2017JM8034);解放军总医院第八医学中心课题基金项目(编号:2016MS-016)
Last Update: 1900-01-01