|Table of Contents|

Expressions of Beclin1 and LC3B in tissues of gastric adenocarcinoma with different differentiation degrees and associations of Beclin1，LC3B with Hp infection

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 01
Page:
83-88
Research Field:
Publishing date:

Info

Title:
Expressions of Beclin1 and LC3B in tissues of gastric adenocarcinoma with different differentiation degrees and associations of Beclin1，LC3B with Hp infection
Author(s):
Li Changxin1; Zhou Tao2; Zheng Jiao1; Meng Sihong1; Wang Jia1; Li Qinna1
1.Department of Pathology，Beijing Traditional Chinese Medical Hospital of Shunyi District，Beijing 101300，China；2.Digestive Center，Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University，Beijing 100010，China.
Keywords:
Beclin1; LC3B; gastric adenocarcinoma; Hp
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2020.01.021
Abstract:
Objective：To observe the expression of Beclin1 and LC3B in the gastric adenocarcinomas with different differentiation degree tissues and the association of Beclin1，LC3B with Hp infection.Methods：HE and immunohistochemistry stainings were used to detect the expression of Beclin1，LC3B and Hp in the gastric adenocarcinoma tissues(87 cases)and in the chronic non-atrophic gastritis tissues surrounding the gastric adenocarcinomas(87 cases)．Results：The high level expression rates of Beclin1，LC3B in the gastric adenocarcinoma group were 64.4%(56/87)，62.1%(54/87)respectively，and compared with those in the chronic non-atrophic gastritis group，there were statistically significant differences respectively(P<0.001，P<0.001).Compared with those of Beclin1，LC3B in the poorly differentiated adenocarcinom group［（47.8%(11/23),30.4%（7/23）］，the high level expression rates of Beclin1，LC3B in the well-moderately differentiated adenocarcinoma group［70.3%(45/64),73.4%（47/64）］ were higher，and there were statistically significant differences respectively (P<0.05，P<0.05).Immunohistochemistry showed that there was significantly positive correlation between the dot shaped positive expression of LC3B and the high level expression of LC3B in the corresponding gastric adenocarcinoma(r=0.479，P<0.001).The expression of Beclin1 in the gastric adenocarcinomas was positively related with the expression of LC3B(r=0.408，P<0.001).The high level expression rates of Beclin1，LC3B in the Hp(+)gastric cancer tissues (79.59%，75.51%)were significantly higher than that in the Hp(-)gastric cancer tissues(44.74%，44.74%)，and there were significant differences between them(P<0.05,P<0.05).There were significantly positive correlations between the Hp positive expression and the high level expression rates of Beclin1，LC3B in the gastric adenocarcinoma tissues(r=0.286，P<0.05；r=0.298，P<0.05).Conclusion：The increased expression of Beclin1，LC3B in the gastric adenocarcinoma tissues，especially in the well-moderately differentiated adenocarcinoma tissues，suggested that Beclin1，LC3B participated in the procession of the gastric epithelial cells' malignant transformation，progression and differentiation.The result of Hp infections significantly correlating with the high level expression rates of Beclin1，LC3B in the gastric adenocarcinoma tissues pointed out that Hp infections could induced autophagy activity increasing and they interacted with each other which could effect the development of the gastric adenocarcinoma.

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北京市卫生系统高层次卫生技术人才培养计划项目资助（编号：2015-3-114）
Last Update: 1900-01-01