|Table of Contents|

Effect of microRNA-224 on apoptosis of colon cancer cells induced by hypoxia

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 01
Page:
30-33
Research Field:
Publishing date:

Info

Title:
Effect of microRNA-224 on apoptosis of colon cancer cells induced by hypoxia
Author(s):
Zhang Juan1; Feng Yan2; He Shuixiang1
1.Department of Gastroenterology，the First Affiliated Hospital of Xi’an Jiaotong University，Shaanxi Xi’an 710061，China；2.Department of Gastroenterology，People’s Hospital of Xinjiang Uygur Autonomous Region，Xinjiang Urumqi 830001，China.
Keywords:
microRNA-224; colon cancer cells; hypoxia; apoptosis
PACS:
R735.3+5
DOI:
10.3969/j.issn.1672-4992.2020.01.008
Abstract:
Objective：To detect the role of microRNA-224 in the occurrence and development of colon cancer by colon cancer cell experiment.Methods：After the apoptosis model of colon cancer cells caused by hypoxia was established，we used Cell Counting Kit-8 to analyse the cell viability in hypoxia colon cancer cells group(hypoxia group)and normal colon cancer cells group(control group).Flow cytometry(FCM) was used to detect the rate of apoptosis in hypoxia colon cancer cells group (hypoxia group) and colon cancer cells group(control group)，also detect the rate of apoptosis in microRNA-224-transfected colon cancer cells group(microRNA-224 group) and Vector-NC control group.Western blot was used to detect the proteins of apoptosis Caspase-3 in microRNA-224-transfected colon cancer cells group (microRNA-224 group) and Vector-NC control group.Results：After treatment with hypoxia in colon cancer cells，the apoptotic rate of cells was significantly higher than the control group(P<0.05).After pre-transfecting with microRNA-224，the apoptotic rate of cells was lower in hypoxia-treatment colon cancer cells than Vector-NC control group(P<0.05).The apoptotic protein Caspase-3 in microRNA-224-transfected colon cancer cells was lower than Vector-NC control group(P<0.05).Conclusion：MicroRNA-224 can inhibit the apoptosis of colon cancer cells，which has significant influence in the development of colon cancer.

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