|Table of Contents|

The effect of miR-139-5p on proliferation and apoptosis of breast cancer cells by targeting Notch signal pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2020 01
Page:
22-26
Research Field:
Publishing date:

Info

Title:
The effect of miR-139-5p on proliferation and apoptosis of breast cancer cells by targeting Notch signal pathway
Author(s):
Guo Qiang; Liao Xinwei
Department of General Surgery，Zhumadian Central Hospital，Henan Zhumadian 463000，China.
Keywords:
breast cancer; miR-139-5p; apoptosis; Notch
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2020.01.006
Abstract:
Objective：To investigate the effect of miR-139-5p on cell proliferation and apoptosis in breast cancer via targeting Notch signal pathway.Methods：The level of miR-139-5p and Notch1 in breast cancer cells and mammary epithelial cells was evaluated by RT-qPCR.Dual luciferase reporter gene assay was applied to analyze the regulation effect of miR-139-5p on 3’ UTR of Notch1.Furthermore，CCK8 and flow cytometry assay were used to determine the proliferation and apoptosis of MDA-MB-231 cells，respectively.Finally，the protein expression of Notch1 and its related apoptotic protein were measured by Western blot assay under the regulation of miR-139-5p.Results：Compared with mammary epithelial cells，the level of miR-139-5p in breast cancer cells was significantly decreased(P<0.05)，especially in MDA-MB-231 cells(P<0.01).miR-139-5p could bind to specific sequence of Notch1 3’ UTR.Additionally，overexpression of miR-139-5p could significantly attenuate the cell viability and promote the apoptosis of MDA-MB-231 cells(P<0.01).miR-139-5p was capable of inhibiting the protein expression of Notch1 and resulted in decreased expression of Bcl-2 and increased expression of Bax(P<0.01).Conclusion：The mechanism of miR-139-5p on the proliferation and apoptosis of breast cancer cells was mediated by targeting Notch signal pathway.

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河南省卫生和计划生育委员会支助项目（编号：201700320）
Last Update: 1900-01-01