|Table of Contents|

Expression of dipeptidase 1 in colorectal intraepithelial neoplasia and its clinical significance

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 06
Page:
1024-1028
Research Field:
Publishing date:

Info

Title:
Expression of dipeptidase 1 in colorectal intraepithelial neoplasia and its clinical significance
Author(s):
Huang Jinhua; Wen Xiaoping; Zeng Jianqiang; Zou Ping; Yuan Lei; Xiao Youhua
Ganzhou Cancer Hospital,Jiangxi Ganzhou 341000,China.
Keywords:
colorectal intraepithelial neoplasia; adenocarcinoma; normal colorectal mucosa epithelial tissue; dipeptidase 1
PACS:
R735.3+5;R735.3+7
DOI:
10.3969/j.issn.1672-4992.2019.06.029
Abstract:
Objective:To investigate the expression of dipeptidase 1 (DPEP1) mRNA and protein in colorectal intraepithelial neoplasia and its relationship with clinical characteristics.Methods:Real-time fluorescence quantitative reverse transcription PCR(RFQ-RT-PCR) was used to detect the expressions of DPEP1 mRNA in a total of 60 cases of colorectal intraepithelial neoplasia (35 cases of adenomas and high-grade intraepithelial neoplasia,25 cases of adenoma and low level intraepithelial neoplasia),and 30 cases of normal colorectal mucosa epithelial tissues and 20 cases of well-differentiated adenocarcinomas,as well as their relationships with clinicopathological features were analyzed.Immunohistochemistry was used to detect the distribution and expression of DPEP1 protein.Results:The expression levels of DPEP1 mRNA in the high level traepithelial neoplasia group and low level intraepithelial neoplasia group were 0.560±0.052 and 0.328±0.040 respectively (P=0.001).The expression levels of DPEP1 mRNA in 60 cases of colorectal intraepithelial neoplasia,30 cases of normal colorectal mucosa epithelial tissues and 20 cases of well-differentiated adenocarcinomas were 0.684±0.108,0.235±0.045 and 0.832±0.136 respectively (P＜0.001).The expression rate of DPEP1 in well-differentiated adenocarcinomas of was 100%(20/20),significantly higher than the 83.33%（50/60） in colorectal intraepithelial neoplasia and 20%(6/30) in normal colorectal mucosa epithelial tissue,diffusely distributing in the cytoplasm (P＜0.01).The positive expression rate of DPEP1 in the high level traepithelial neoplasia group and low level intraepithelial neoplasia group were 88.57%(31/35),76%(19/25)respectively.There were correlation between DPEP1 expression level and intraepithelial neoplasia pathologic stage and advance (P＜0.05)，but there were no correlation with gender and age (P＞0.05).Conclusion:DPEP1 is expressed in the colorectal intraepithelial neoplasia and early stage colorectal carcinoma and affects tumor cell invasiveness，associated with the advance of patients with tumor.

References:

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