|Table of Contents|

Expression and clinical value of FOXO1 in renal cell carcinoma tissues and cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 06
Page:
1016-1020
Research Field:
Publishing date:

Info

Title:
Expression and clinical value of FOXO1 in renal cell carcinoma tissues and cells
Author(s):
Zhou Libin; Li Xing; Li Sheng; Wu Tielin; Long Huimin
Department of Urology,Ningbo City Medical Treatment Center Lihuili Hospital,Zhejiang Ningbo 315040,China.
Keywords:
FOXO1; renal cell carcinoma; renal cell carcinoma cell lines; cell proliferation
PACS:
R737.11
DOI:
10.3969/j.issn.1672-4992.2019.06.027
Abstract:
Objective:To study FOXO1 in renal cell carcinoma (RCC) tissues and renal cell carcinoma cell lines and its relation with renal carcinogenesis.Methods：The expression levels of FOXO1 transcripts and protein in renal cell carcinoma cell lines were examined by quantitative Real-time PCR and Western blot.Immunohistochemistry was used to examined FOXO1 protein in renal cell carcinoma tissues.769-P cells were transfected with small interference RNA against human FOXO1 gene.CCK8 assay was used to estimate the cell growth.Results：The FOXO1 protein positive rates in clear cell RCC (ccRCC) and papillary RCC (pRCC) were lower than chromophobe RCC (chRCC) (P＜0.05).The expression levels of FOXO1 mRNA and protein in 769-P cells were higher than those in 786-O and Caki-1 cells.After inhibiting the expression levels of FOXO1 mRNA and protein using specific siRNA,the growth of 769-P cells was increased.Conclusion：The decreased expression of FOXO1 had correlated not only with the subtypes of RCC but also with the stage and grade of ccRCC.FOXO1 may become a diagnostic and prognostic marker and a new target to therapy the renal cell carcinoma.

References:

［1］Katoh M.Human FOX gene family (review)［J］.Int J Oncol,2004,25(5):1495-1500.
［2］Kim CG,Lee H,Gupta N,et al.Role of Forkhead Box Class O proteins in cancer progression and metastasis ［J］.Semin Cancer Biol,2018,50:142-151.
［3］Chow WH,Devesa SS,Warren JL,et al.Rising incidence of renal cell cancer in the United States ［J］.JAMA,1999,281(17):1628-1631.
［4］Burgering BM,Kops GJ.Cell cycle and death control:Long live Forkheads ［J］.Trends Biochem Sci,2002,27(7):352-360.
［5］Maiese K,Chong ZZ,Shang YC,et al.A "FOXO" in sight:targeting Foxo proteins from conception to cancer ［J］.Med Res Rev,2009,29(3):395-418.
［6］Burgering BM,Medema RH.Decisions on life and death:FOXO Forkhead transcription factors are in command when PKB/Akt is off duty ［J］.J Leukoc Biol,2003,73(6):689-701.
［7］Myatt SS,Wang J,Monteiro LJ,et al.Definition of microRNAs that repress expression of the tumor suppressor gene FOXO1 in endometrial cancer ［J］.Cancer Res,2010,70(1):367-377.
［8］Guttilla IK,White BA.Coordinate regulation of FOXO1 by miR-27a,miR-96,and miR-182 in breast cancer cells ［J］.J Biol Chem,2009,284(35):23204-23216.
［9］Yang Y,Blee AM,Wang D,et al.Loss of FOXO1 cooperates with TMPRSS2-ERG overexpression to promote prostate tumorigenesis and cell invasion ［J］.Cancer Res,2017,77(23):6524-6537.
［10］Zhao Z,Zhang M,Duan X,et al.TRPM7 regulates AKT/FOXO1-dependent tumor growth and is an independent prognostic indicator in renal cell carcinoma ［J］.Mol Cancer Res,2018,16(6):1013-1023.
［11］Gan B,Lim C,Chu G,et al.FoxOs enforce a progression checkpoint to constrain mTORC1-activated renal tumorigenesis ［J］.Cancer Cell,2010,18(5):472-484.
［12］Choi Y,Park J,Ko YS,et al.FOXO1 reduces tumorsphere formation capacity and has crosstalk with LGR5 signaling in gastric cancer cells ［J］.Biochem Biophys Res Commun,2017,493(3):1349-1355.
［13］Wang J，Sun G.FOXO1-MALAT1-miR-26a-5p feedback loop mediates proliferation and migration in osteosarcoma cells ［J］.Oncol Res,2017,25(9):1517-1527.
［14］Zhang Y,Jia L,Ji W,et al.Higher expression of FOXOs correlates to better prognosis of bladder cancer ［J］.Oncotarget,2017,8(56):96313-96322.
［15］Kojima T,Shimazui T,Horie R,et al.FOXO1 and TCF7L2 genes involved in metastasis and poor prognosis in clear cell renal cell carcinoma ［J］.Genes Chromosomes Cancer,2010,49(4):379-389.
［16］Goto T,Takano M,Albergaria A,et al.Mechanism and functional consequences of loss of FOXO1 expression in endometrioid endometrial cancer cells ［J］.Oncogene,2008,27(1):9-19.

Memo

Memo:
浙江省医药卫生科技计划项目（编号：2019KY603）;宁波市自然基金（编号：2017A610186）
Last Update: 1900-01-01