|Table of Contents|

Growth inhibiting and promoting apoptosis of Trillium saponins on xenografts of breast cancer cells MDA-MB-231 in nude mice

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 06
Page:
952-955
Research Field:
Publishing date:

Info

Title:
Growth inhibiting and promoting apoptosis of Trillium saponins on xenografts of breast cancer cells MDA-MB-231 in nude mice
Author(s):
Zeng Xiaocong1; Zhang Yuanqi2; Huang Shengchao2; Yu Liyan2; Yan Zeming2; Sun Yang3; Chen Xiaodong2; Li Jianwen2
1.Department of Surgical Oncology，No.422 Hospital of PLA，Guangdong Zhanjiang 524005,China；2.Department of Vascular,Thyroid and Breast Surgery,Affiliated Hospital of Guangdong Medical University,Guangdong Zhanjiang 524001,China;3.Department of Pharmacology，School of Pharmacy,the Fourth Military Medical University,Shaanxi Xi'an 710032,China.
Keywords:
Trillium saponins; proliferation; apoptosis; breast cancer
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2019.06.011
Abstract:
Objective:To investigate the effects of Trillium saponins on the growth of xenografts of MDA-MB-231 in nude mice and the possible mechanisms.Methods:MDA-MB-231 cells were cultured and inoculated subcutaneously to the back of BALB/c nude mice.Five days later,the nude mice were intraperitoneally injected with Trillium saponins.Tumor volumes and body weight were measured every 3 days to the end of the experiment.TUNEL assay was used to detect the apoptosis of tumor cells.Western blot was employed to detect the expression of apoptosis related protein caspase-3,8,9.Results:Trillium saponins could inhibit the growth of xenografts of MDA-MB-231 significantly.At the end of the experiment,the tumor volumes were as follows:model group(1142.24±164.32)mm3,Trillium saponins(5 mg/kg)(552.90±49.71)mm3,and Trillium saponins(10 mg/kg)(269.78±48.84)mm3.Trillium saponins could induce apoptosis of tumor cells and promote expression of cleaved caspase-3,9 in a dose-dependent manner.Conclusion:Trillium saponins suppressed the growth of xenografts of MDA-MB-231,at least in part,by inducing apoptosis of tumor cells through a caspase-dependent way.

References:

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