|Table of Contents|

IFN-γ facilitates malignant progression in PTC cell line by inducing EMT

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 06
Page:
947-951
Research Field:
Publishing date:

Info

Title:
IFN-γ facilitates malignant progression in PTC cell line by inducing EMT
Author(s):
Lv Nannan1; 2; Gao Yun2; Shan Zhongyan2
1.Department of Endocrinology,The 4th People's Hospital of Shenyang,Liaoning Shenyang 110034,China;2.The Endocrine Institute and the Liaoning Provincial Key Laboratory of Endocrine Diseases,The First Hospital of China Medical University,Liaoning Shenyang 110001,China.
Keywords:
interferon gamma; invasion and metastasis; papillary thyroid cancer; epithelial mesenchymal transition
PACS:
R736.1
DOI:
10.3969/j.issn.1672-4992.2019.06.010
Abstract:
Objective:To investigate the effects of IFN-γ on the migration and invasion of the PTC cell line TPC-1,confirmed whether the epithelial-mesenchymal transition (EMT) mechanism was involved in the progression.Methods:The effect of IFN-γ on cell migration and invasion was assessed by Wound-healing and Transwell assay.The expression level of mRNA and protein of EMT makers E-cadherin,N-cadherin and Vimentin was analyzed using RT-PCR and Western blot,and their distributions were detected by immunofluorescence.Results:The Wound-healing and Transwell showed that the migratory and invasive abilities of PTC cell treated with IFN-γ were increaseed (P＜0.05).RT-PCR showed the decreased mRNA level of E-cadherin and the increased mRNA of Vimentin,but there was no obvious increase in mRNA of N-cadherin.Western blot showed that E-cadherin protein expression was decreased,N-cadherin and Vimentin were increased (P＜0.05).These results were further confirmed by immunefluorescence analysis.Conclusion:Our results showed that EMT involved in the malignant progression induced by IFN-γ in PTC cell,imply that inflamemation participated in invasion and metastasis of human papillary thyroid cancer.

References:

［1］Lim H,Devesa SS,Sosa JA,et al.Trends in thyroid cancer incidence and mortality in the United States,1974-2013［J］.JAMA,2017,317(13):1338-1348.
［2］Liu Yuqin,Zhang Shuquan,Chen Wanqing,et al.Trend of incidence and mortality on thyroid cancer in China during 2003-2007［J］.Chin J Epidemiol,2012,33(10):1044-1048.［刘玉琴,张书全,陈万青，等.中国2003-2007年甲状腺癌发病死亡现状及流行趋势分析［J］.中华流行病学杂志,2012,33(10):1044-1048.］
［3］Carter WB,Tourtelot JB,Savell JG,et al.New treatments and shifting paradigms in differentiated thyroid cancer management［J］.Cancer Control,2011,18(2):96-103.
［4］Meggy Suarez Carmona,Julien Lesage.EMT and inflammation:Inseparable actors of cancer progression［J］.Mol Oncol,2017,11(7):805-823.
［5］Tsukasa Shibue,Robert A.Weinberg.EMT,CSCs,and drug resistance:The mechanistic link and clinical implications［J］.Nat Rev Clin Oncol,2017,14(10):611-629.
［6］Grivennikov SI,Greten FR,and Karin M.Immunity,inflammation,and cancer［J］.Cell,2010,140(6):883-899.
［7］Morten Andersen,Zamra Sajid,Rasmus K Pedersen,et al.Mathematical modelling as a proof of concept for MPNs as a human inflammation model for cancer development［J］.PLoS One,2017,12(8):e0183620.
［8］Kalluri R,Weinberg RA.The basics of epithelial-mesenchymal transition［J］.J Clin Invest,2009,119:1420-1428.
［9］Patricia G Santamaria,Gema Moreno Bueno.EMT:Present and future in clinical oncology［J］.Mol Oncol,2017,11(7):718-738.
［10］Sarah Heerboth,Genevieve Housman,Meghan Leary,et al.EMT and tumor metastasis［J］.Clin Transl Med,2015,4:6.
［11］Pestka S,Krause CD,Walter MR.Interferons,interferon-like cytokines,and their receptors［J］.Immunol Rev,2004,202:8-32.
［12］Hyeon Joo Cheon,Ernest C Borden，George R Stark.Interferons and their stimulated genes in the tumor microenvironment［J］.Semin Oncol,2014,41(2):156-173.
［13］Lou Y,Diao L,Cuentas ER,et al.Epithelial-mesenchymal transition is associated with a distinct tumor microenvironment including elevation of inflammatory signals and multiple immune checkpoints in lung adenocarcinoma［J］.Clin Cancer Res,2016,22,3630-3642.
［14］Lei Wang,Yinghua Zhao,Yi Liu,et al.IFN-γ and TNF-α synergistically induce mesenchymal stem cell impairment and tumorigenesis via NF-κB signaling［J］.Stem Cells,2013,31(7):1383-1395.
［15］Visciano C,Liotti F,Prevete N,et al.Mast cells induce epithelial-to-mesenchymal transition and stem cell features in human thyroid cancer cells through an IL-8-Akt-Slug pathway［J］.Oncogene,2015,34,5175-5186.
［16］Yuliya I Petrova,Leslayann Schecterson,Barry M Gumbiner.Roles for E-cadherin cell surface regulation in cancer［J］.Mol Biol Cell,2016,27(21):3233-3244.
［17］Liu Z,Kakudo K,Bai Y,et al.Loss of cellular polarity/cohesiveness in the invasive front of papillary thyroid carcinoma,a novel predictor for lymph node metastasis，possible morphological indicator of epithelial mesenchymal transition［J］.J Clin Pathol,2011,64:325-329.
［18］Wu S,Sun G,Huang C,et al.Tumor necrosis factor-α induces epithelial-mesenchymal transition of renal cell carcinoma cells via a nuclear factor kappa B-independent mechanism［J］.Exp Biol Med,2011,236:1022-1029.
［19］Wang H,Wang H,Zhou B,et al.Epithelial-mesenchymal transition (EMT) induced by TNF-a requires AKT/GSK-3b-mediated stabilization of snail in colorectal cancer［J］.PLoS One,2013,8(2):e56664.

Memo

Memo:
辽宁省自然科学基金指导计划（编号：20180550452）
Last Update: 1900-01-01