|Table of Contents|

miRNA-369 inhibited osteosarcoma proliferation and induced apoptosis by targeting SOX4

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 06
Page:
939-942
Research Field:
Publishing date:

Info

Title:
miRNA-369 inhibited osteosarcoma proliferation and induced apoptosis by targeting SOX4
Author(s):
Zhang Yi; Li Jiazhen; Zhang Yan; Lu Xinchang; Wu Xuejian
Department of Orthopedics,the First Affiliated Hospital of Zhengzhou University,Henan Zhengzhou 450052,China.
Keywords:
osteosarcoma; miR-369; SOX4; Bcl-2; proliferation; apoptosis
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2019.06.008
Abstract:
Objective:To investigate the effects of microRNA-369 on the proliferation and apoptosis against osteosarcoma MG-63 cell through SOX4.Methods:The expression of miR-369 in osteosarcoma MG-63 cell was detected by Real-time PCR after miR-369mimics transfection.After the validation of miR-369 over-expression,CCK-8 and flow cytometry assay were used to detect the proliferation and apoptosis rate.Then the expression levels of SOX4 mRNA were detected by Real-time PCR.The relationship between the miR-369 and SOX4 was investigated by dual luciferase assay.The expression of SOX4 and Bcl-2 related proteins were detected by Western-blot.Results:After miR-369 mimics transfection in MG-63 cell,relative expression of miR-369 in mimics group was significantly increased compared with the negative-control group(P=0.009).The proliferation at 24 h and 48 h after miR-369 mimics transfection were significantly suppressed compared with the NC group.The flow cytometry assay suggested the apoptosis cell was increased in mimic group compared with the NC group (P=0.031).The expressions of SOX4 in the mRNA and protein levels were reduced significantly in mimics group while the expression of Bcl-2 related proteins were regulated significantly after transfection.Conclusion:miR-369 inhibited osteosarcoma MG-63 cell proliferation and induced apoptosis by targeting SOX4.

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Memo

Memo:
河南省科技计划项目（编号：182102310370）；河南省基础与前沿技术研究计划项目（编号：142300410400）
Last Update: 1900-01-01