|Table of Contents|

The effect of Galectin-3 regulated Wnt signaling pathway on apoptosis of human glioma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 06
Page:
934-938
Research Field:
Publishing date:

Info

Title:
The effect of Galectin-3 regulated Wnt signaling pathway on apoptosis of human glioma cells
Author(s):
You YuLi XiaoxuWang ZhongrenZhao MingXu XinHan LeiZhao Sijun
Department of Neurosurgery,Affiliated Tumor Hospital of Zhengzhou University (Henan Cancer Hospital),Henan Zhengzhou 450008,China.
Keywords:
Galectin-3Wnt signaling pathwaygliomaapoptosis
PACS:
R730.264
DOI:
10.3969/j.issn.1672-4992.2019.06.007
Abstract:
Objective:To investigate the effect of Galectin-3 regulated Wnt signaling pathway on apoptosis of human glioma cells.Methods:The mRNA and protein expression of Galectin-3 in human glioma tissue were detected by RT-PCR and Western blot.Expression of Galectin-3 protein in human glioma U251,U87 and SHG-44 cells were detected by Western blot.Galectin-siRNA was transfected into human glioma U87 cells for 48 h.The expression of Galectin-3,Wnt5a,β-catenin and Cleaved Caspase3 protein and cell apoptosis were detected by Western blot and flow cytometry.Results:The expression of Galectin-3 mRNA and protein in human glioma tissue were significantly higher than those in the tumor adjacent tissues (P<0.01).Galectin-3 protein expression in U251,U87,SHG-44 cells from high to low as U87>U251>SHG-44.U87 cells was selected as a follow-up study.Galectin-3 protein in Galectin-3-siRNA1 was lowest,selected as a follow-up study.The apoptosis rate,Cleaved Caspase3,Wnt5a,β-catenin protein expression in NC-siRNA group and the control group had no significant difference (P>0.05).Compared with control group,the apoptosis rate in Galectin-3-siRNA group were significantly higher.Cleaved Caspase3 protein expressionwere significantly higher,and the expression of Wnt5a and β-catenin protein was significantly lower than the control group (P<0.01).Conclusion:Silencing of Galectin-3 expression can induce apoptosis of human glioma cells,which may be related to the downregulation of Wnt signaling pathway.

References:

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河南省医学科技攻关计划项目(编号:201503189)
Last Update: 1900-01-01