|Table of Contents|

Inhibitory effects of lncRNA AK126393 on colon cancer cells and related mechanisms

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 06
Page:
924-928
Research Field:
Publishing date:

Info

Title:
Inhibitory effects of lncRNA AK126393 on colon cancer cells and related mechanisms
Author(s):
Zhuang Biao; Min Zhijun; Ni Xiong; Wang Tingfeng; Wu Dejun; Cui Peng
General Surgery Department,Shanghai Pudong Hospital,Shanghai 201399,China.
Keywords:
long chain non coding RNA-AK126393; colon cancer; proliferation; apoptosis; autophagy
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2019.06.005
Abstract:
Objective:To explore the role and mechanism of long chain non coding RNA (lncRNA) AK126393 in the regulation of the apoptosis of human colon cancer cells.Methods:The expressions of lncRNA AK126393 in human normal colonic epithelial cells and different colon cancer cell lines were detected by quantitative real-time polymerase chain reaction (qRT-PCR),and pcDNA3.1-AK126393 was used to transfect the HCT116 cell.The cell viability was detected by MTT assay,and the percentage of apoptosis was detected by flow cytometry,and the expressions of the target proteins in the cells were measured by Western blotting.Results:The expressions of AK126393 in human colon cancer cell lines were significantly lower than that of human immortalized colonic epithelial cell linenormal colonic epithelial cell line NCM460.The expression of AK126393 in HCT116 cells was the lowest,and the relative expression level of AK126393 was significantly increased after pcDNA3.1-AK126393 transfected into HT116 cells.The overexpression of AK126393 inhibited the proliferation of HCT116 cells,promoted apoptosis in HCT116 cells and induced the occurrence of autophagy,while the overexpression of AK126393 inhibited the phosphorylation of AKT/ERK1/2 signaling pathway in the HCT116 cells.Conclusion:lncRNA AK126393 can inhibit the proliferation of colon cancer cells,promote the apoptosis and induce the occurrence of autophagy in colon cancer cells,which may be achieved by inhibiting the activation of AKT/ERK1/2 signaling pathway.

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