|Table of Contents|

Effects of different doses of radiation on the proliferation of alveolar macrophages in rats

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 06
Page:
911-914
Research Field:
Publishing date:

Info

Title:
Effects of different doses of radiation on the proliferation of alveolar macrophages in rats
Author(s):
Wang Jiawei; Wang Xuekai; Hou Wei
Guang'anmen Hospital of China Academy of Chinese Medical Sciences，Beijing 100053,China.
Keywords:
radiation-induced lung injury; rat alveolar macrophage; proliferation ability
PACS:
R730.55
DOI:
10.3969/j.issn.1672-4992.2019.06.002
Abstract:
Objective:To observe the effect of different doses of radiation on the proliferation of the cells,and to lay a foundation for the establishment of the radiation lung injury cell model.Methods:The alveolar macrophages of rats were irradiated with different doses of radiation (0 Gy,2 Gy,4 Gy,6 Gy) by high energy linear accelerator (X ray).The morphology of the cells under the cell microscope was observed after 24 h,48 h and 72 h after irradiation,and the cell proliferation ability was detected by MTT method.Results：The number of cells in the irradiated group was less than that in the normal group (group 0 Gy) at the different time nodes.In the form of cell morphology,the cells in the 0 Gy group showed a series of growth characteristics of the alveolar macrophages in the rats,while the cells in the irradiated group showed a single dispersed growth.After the MTT test,the measured values of each time node in 0 Gy were 0.139,0.165,0.205 and 0.112,0.127,0.151 in 2 Gy and 0.109,0.121,0.138 in 4 Gy,and 0.088,0.094,0.099 in 6 Gy.Compared with the normal group,the absorbance values of different doses were different,P=0.000<0.05.In 24 h,48 h,72 h,the absorbance of each group were different,P=0.000<0.05.The difference between group 2 Gy and 4 Gy group was not obvious,P=0.052>0.05.There was a significant difference between the groups in 6 Gy group and other two.Conclusion:The radiation can affect the growth and proliferation of alveolar macrophages in rats,and disappears the characteristics of the growth of the rat alveolar macrophages,in which the 6 Gy dose rays have the greatest impact on the cells.

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Memo

Memo:
National Natural Science Foundation of China(No.81673754);国家自然科学基金面上项目（编号：81673754）
Last Update: 1900-01-01