|Table of Contents|

The effect of ginsenoside Rh2 on neuroblastoma cell line SH-SY5Y proliferation,apoptosis,migration and invasion

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 06
Page:
907-910
Research Field:
Publishing date:

Info

Title:
The effect of ginsenoside Rh2 on neuroblastoma cell line SH-SY5Y proliferation,apoptosis,migration and invasion
Author(s):
Xia Baojia; Zhang Mingjie; Da Zuke; Zhou Difan; Zhao Hongyu
Department of Neurosurgery,Shengjing Hospital of China Medical University,Liaoning Shenyang 110004，China.
Keywords:
neuroblastoma; ginsenoside Rh2; matrix metalloproteinase-2; cell invasion/metastasis; wnt signaling pathway
PACS:
R739.4
DOI:
10.3969/j.issn.1672-4992.2019.06.001
Abstract:
Objective:To investigate the characteristics of early metastasis of neuroblastoma (NB)，the effects of ginsenoside Rh2 on proliferation,apoptosis,migration and invasion of neuroblastoma were studied.Methods：Ginsenoside Rh2 was used to interfere with neuroblastoma cell line SH-SY5Y (5,10,20,40,80 μg/ml).Cell counting kit-8 (CCK-8) assay was used to detect cell proliferation,cell scratch test was used to detect cell migration ability,transwell cell model was used to detect cell invasiveness,and Western-blot test was used to detect the expression of matrix metalloproteinase 2 (MMP2) Bax,Bcl-2,and β-catenin protein in neuroblastoma cells under different concentrations.Results：Ginsenoside Rh2 can inhibit the proliferation,migration and invasion of neuroblastoma in time and dose dependent manner to reduce the expression of migration related protein MMP2.The level of β-catenin in neuroblastoma cells treated with ginsenoside Rh2 decreased.Conclusion：Ginsenoside Rh2 can inhibit the proliferation of neuroblastoma cells in vitro,and down regulation of MMP2 protein to inhibit the migration and invasion of tumor cells,and inhibit cell growth through wnt pathway.

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辽宁省自然科学基金(编号：2015020510)
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