|Table of Contents|

The effect of specific silencing EPB49 on cell metastasis of colorectal cancer and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 05
Page:
761-766
Research Field:
Publishing date:

Info

Title:
The effect of specific silencing EPB49 on cell metastasis of colorectal cancer and its mechanism
Author(s):
Jiang Shengchang1Wang Xiaolei2
1.Department of General Surgery,Xinyang Central Hospital,Henan Xinyang 464000,China;2.Department of General Surgery,the First Affiliated Hospital of Henan University,Henan Kaifeng 475000,China.
Keywords:
colorectal cancersiRNAEPB49JAK2/STAT3
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2019.05.012
Abstract:
Objective:To investigate the effect of small interfering RNA(siRNA) silencing erthrocyte brane protein band 4.9(EPB49)on proliferation,invasion and migration of colorectal cancer SW480 cells and its possible mechanism.Methods:Colorectal cancer SW480 cells were divided into blank control group,NC control group(transfected negative control siRNA-NC),siRNA-EPB49 group.siRNA-NC and siRNA-EPB49 were transfected into SW480 cells by Lipofectamine 2000.The proliferation activity of the cells was detected by CCK-8 experiment.Transwell assay was used to detect the invasion and migration of SW480 cells.Western Blot was used to detect the expression STAT3(p-STAT3),just another kinase 2(JAK2),and phosphated JAK2(p-JAK2) protein.Results:After silencing EPB49 expression,the expression of EPB49 protein in siRNA-EPB49 group was significantly decreased than that in blank control group(P<0.05),and the cell proliferation activity,invasion and migration ability increased significantly(P<0.05).The results of Western Blot showed that the expression of p-STAT3 and p-JAK2 in SW480 cells increased significantly after EPB49 silenced(P<0.05).Conclusion:Silencing EPB49 can promote the proliferation,invasion and migration of colorectal cancer cells,which is related to the JAK2/STAT3 signaling pathway.

References:

[1]Guend H,Widmar M,Patel S,et al.Developing a robotic colorectal cancer surgery program:Understanding institutional and individual learning curves[J].Surgical Endoscopy,2017,31(7):2820-2828.
[2]Itatani Y,Kawada K,Inamoto S,et al.The role of chemokines in promoting colorectal cancer invasion/metastasis[J].International Journal of Molecular Sciences,2016,17(5):643.
[3]Haase G,Gavert N,Brabletz T,et al.The Wnt target gene L1 in colon cancer invasion and metastasis[J].Cancers,2016,8(5):48.
[4]Pishesha N,Thiru P,Shi J,et al.Transcriptional divergence and conservation of human and mouse erythropoiesis[J].Proceedings of the National Academy of Sciences,2014,111(11):4103-4108.
[5]Chang X,Rong C,Chen Y,et al.(-)-Epigallocatechin-3-gallate attenuates cognitive deterioration in Alzheimer's disease model mice by upregulating neprilysin expression[J].Experimental Cell Research,2015,334(1):136-145.
[6]Murtaza M,Caldas C.Nucleosome mapping in plasma DNA predicts cancer gene expression[J].Nature Genetics,2016,48(10):1105.
[7]Chung CH,Guthrie VB,Masica DL,et al.Genomic alterations in head and neck squamous cell carcinoma determined by cancer gene-targeted sequencing[J].Annals of Oncology,2015,26(6):1216-1223.
[8]Catarinella M,Monestiroli A,Escobar G,et al.IFNα gene/cell therapy curbs colorectal cancer colonization of the liver by acting on the hepatic microenvironment[J].EMBO Molecular Medicine,2016,8(2):155-170.
[9]Massagué J,Obenauf AC.Metastatic colonization by circulating tumour cells[J].Nature,2016,529(7586):298-306.
[10]Farahani E,Patra HK,Jangamreddy JR,et al.Cell adhesion molecules and their relation to (cancer) cell stemness[J].Carcinogenesis,2014,35(4):747-759.
[11]Brown GT,Murray GI.Current mechanistic insights into the roles of matrix metalloproteinases in tumour invasion and metastasis[J].The Journal of Pathology,2015,237(3):273-281.
[12]Yajun C,Yuan T,Zhong W,et al.Investigation of the molecular mechanisms underlying postoperative recurrence in prostate cancer by gene expression profiling[J].Experimental and Therapeutic Medicine,2018,15(1):761-768.
[13]Aparicio-Siegmund S,Sommer J,Monhasery N,et al.Inhibition of protein kinase Ⅱ(CK2) prevents induced signal transducer and activator of transcription(STAT) 1/3 and constitutive STAT3 activation[J].Oncotarget,2014,5(8):2131.
[14]Liu X,Ji Q,Ye N,et al.Berberine inhibits invasion and metastasis of colorectal cancer cells via COX-2/PGE2 mediated JAK2/STAT3 signaling pathway[J].PLoS One,2015,10(5):e0123478.
[15]Jhaveri K,Teplinsky E,Silvera D,et al.Hyperactivated mTOR and JAK2/STAT3 pathways:Molecular drivers and potential therapeutic targets of inflammatory and invasive ductal breast cancers after neoadjuvant chemotherapy[J].Clinical Breast Cancer,2016,16(2):113-122.
[16]Lee DH,Sung KS,Bartlett DL,et al.HSP90 inhibitor NVP-AUY922 enhances TRAIL-induced apoptosis by suppressing the JAK2-STAT3-Mcl-1 signal transduction pathway in colorectal cancer cells[J].Cellular Signalling,2015,27(2):293-305.
[17]Gu Lei,Zhang Lijun,Liu Lifang,et al.Inhibitors of JAK/STAT signaling pathway and lung cancer[J].Modern Oncology,2018,26(02):314-317.[谷蕾,张丽军,刘立芳,等.JAK/STAT信号通路抑制剂与肺癌[J].现代肿瘤医学,2018,26(02):314-317.]
[18]Chen HY,Zhang N,Cong L,et al.The clinicopathological significance of p-STAT3 activation and SOCS3 expression in gastric cancer and its effect on prognosis[J].Chongqing Medicine,2014,43(32):4316-4319.[陈海云,张楠,从丽,等.胃癌中p-STAT3活化和SOCS3表达的临床病理意义及其对预后的影响[J].重庆医学,2014,43(32):4316-4319.]
[19]Qiao L,Li XJ,Yang ZY,et al.Expression of STAT3 and p-STAT3 in squamous cell carcinoma of the skin and its relationship with the clinicopathological features of squamous cell carcinoma[J].Journal of Shanxi Medical University,2015,46(12):1207-1210.[乔丽,李新技,杨志勇,等.STAT3 及 p-STAT3 在皮肤鳞状细胞癌中的表达及其与鳞癌临床病理特征的关系[J].山西医科大学学报,2015,46(12):1207-1210.]
[20]Han C,Sun BC,Zhao XL,et al.P-STAT3 studies on the metastatic effect of epithelial mesenchymal transition on colon cancer[J].Chinese Cancer Clinic,2017,44(10):463-468.[韩聪,孙保存,赵秀兰,等.p-STAT3 通过上皮间质转化影响结肠癌的转移性研究[J].中国肿瘤临床,2017,44(10):463-468.]
[21]Yang A,Fan H,Zhao Y,et al.Huaier aqueous extract inhibits proliferation and metastasis of tuberous sclerosis complex cell models through downregulation of JAK2/STAT3 and MAPK signaling pathways[J].Oncology Reports,2016,36(3):1491-1498.
[22]Tao Y,Yang S,Wu Y,et al.MicroRNA-216a inhibits the metastasis of gastric cancer cells by targeting JAK2/STAT3-mediated EMT process[J].Oncotarget,2017,8(51):88870.

Memo

Memo:
河南省医学科技攻关计划项目(编号:201204157)
Last Update: 2019-02-01