|Table of Contents|

Preliminary screening of cisplatin resistant DNA methylation phenotypic markers in gastric cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 05
Page:
754-756
Research Field:
Publishing date:

Info

Title:
Preliminary screening of cisplatin resistant DNA methylation phenotypic markers in gastric cancer
Author(s):
Liu Jie; Peng Wei; Gong Zongyue; Xu Xiaohong; Liu Ying
Hunan Traditional Chinese Medical College，Hunan Zhuzhou 412000，China.
Keywords:
gastric cancer; DNA methylation; cisplatin; drug resistance
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2019.05.010
Abstract:
Objective：To find and identify abnormal methylation genes for cisplatin resistant gastric cancer cells.Methods：Gastric cancer cells and drug-resistant cells as our targets，we used DNA methylation chips to compare and analyze the different expressions of two kinds of DNA methylation cells in order to verify the abnormal methylation genes by means of methylation specific PCR(MS-PCR) technique.Results：The genomic DNA methylation profiles of two cell lines of gastric cancer cells and drug-resistant cells were analyzed by DNA methylation microarray.1 095 methylation loci were identified，and 36 hypermethylation and 14 hypomethylation genes were screened out.Then the methylation of these 50 genes was verified by MS-PCR analysis at the cell level.Finally，14 genes，which were identical to the methylation microarray，were screened out，including 11 hypermethylation and 3 hypomethylation genes.Conclusion：When the cancer cells of gastric cancer occur cisplatin resistance，there is a wide range of DNA methylation modification in the cell genome.

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湖南省教育厅科技处一般项目（编号：15C1074）
Last Update: 2019-02-01