|Table of Contents|

Effects of Wnt7a on hepatocellular carcinoma cell apoptosis,invasion and migration

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 05
Page:
746-749
Research Field:
Publishing date:

Info

Title:
Effects of Wnt7a on hepatocellular carcinoma cell apoptosis,invasion and migration
Author(s):
Lan Lihui1Wang Wei2Shao Yue2Zhao Chenghai2Bu Xianmin1
1.Department of Hepatobiliary and Spleen Surgery,Shengjing Hospital of China Medical University,Liaoning Shenyang 110004,China;2.Department of Pathophysiology,the College of Basic Medical Science of China Medical University,Liaoning Shenyang 110122,China.
Keywords:
hepatocellular carcinomaWnt7aapoptosismigrationinvasion
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2019.05.008
Abstract:
Objective:To explore the role of Wnt7a in hepatocellular carcinoma by investigating Wnt7a expression in hepatocellular carcinoma and analyzing effects of Wnt7a on Hep3B cell viability,apoptosis,migration and invasion.Methods:Immunohistochemistry and Western blot were applied to detect Wnt7a expression in tissues and cells,respectively.Hep3B cells were treated with recombinant Wnt7a protein(rWnt7a).Cell viability was assayed by MTT.Cell apoptosis was examined by flowcytometry.Cell migration and invasion was analyzed by transwell.Results:Wnt7a was expressed in hepatocellular carcinoma tissues in a lower level compared with cancer adjacent tissues.Treatment of Hep3B cells with rWnt7a reduced cell viability,increased cell apoptosis,and decreased cell migration and invasion.Conclusion:Wnt7a inhibits Hep3B cell growth,migration,and invasion,suggesting Wnt7a may play a tumor suppressing role in hepatocellular carcinoma.

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Last Update: 2019-02-01