|Table of Contents|

Inhibition of cardiomyocyte microenvironment on proliferation of K562 cells and NCI-H2452 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 05
Page:
743-745
Research Field:
Publishing date:

Info

Title:
Inhibition of cardiomyocyte microenvironment on proliferation of K562 cells and NCI-H2452 cells
Author(s):
Zhou Yujuan; Wen Qinglian; Liu Zongjunlin; Ni Laichao; Yang Qian; Xiang Zhangqiang
Department of Oncology，Southwest Medical University Affiliated Hospital，Sichuan Luzhou 646000，China.
Keywords:
cardiomyocyte conditioned medium; K562 cells; NCI-H2452 cells; inhibit tumor effect
PACS:
R733.7；R734.3
DOI:
10.3969/j.issn.1672-4992.2019.05.007
Abstract:
Objective：To explore the effect of cardiomyocyte conditioned medium(CMCM) on the proliferation of K562 cells and NCI-H2452 cells，and to explore the tumor specificity of CMCM suppressor and its physicochemical properties.Methods：Primary culture of newborn Wistar rat cardiac muscle cells was established.Cisplatin was used as positive control for CMCM.CCK8 was used to analyze the effect of CMCM on the proliferation of K562 cells and NCI-H2452 cells and HL7702 of normal liver cells in vitro.CMCM was then diluted to different proportions to explore the relationship between dilution concentration and tumor inhibition.CMCM was digested with trypsin and treated at different temperatures to explore the physical and chemical properties of CMCM.Results：The cell survival rate of K562 cells and NCI-H2452 cells was significantly decreased after co-culture with CMCM，compared with the control group(P<0.05)，but there was no significant effect on the normal cells(P>0.05).After trypsin digestion and heat treatment，the activity of myocardial cell culture remained，and the inhibitory effect of CMCM was in a time-and dose-dependent manner.Conclusion：CMCM can inhibit the proliferation of K562 cells and NCI-H2452 cells.The active component of CMCM was found stable under different temperatures and trypsin.The inhibitory effect is time-and dose-dependent manner.

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Memo

Memo:
四川省卫生和计划委员会科研项目（编号：17PJ575）；泸州市科技计划项目（编号：2017LZXNYD-Z04）
Last Update: 2019-02-01