|Table of Contents|

Celastrol inhibits HepG2 cells proliferation and promotes cells apoptosis by targeting lncRNA MEG3

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 05
Page:
739-742
Research Field:
Publishing date:

Info

Title:
Celastrol inhibits HepG2 cells proliferation and promotes cells apoptosis by targeting lncRNA MEG3
Author(s):
Chen Xiaolei1; Yang Xiuli1; Zhou Tianzhu1; Zhou Mengyang1; Li Xinxin1; Zhang Haiyan2; Meng Xin3
1.Institute of Forensic and Judicial Research，Shenyang Medical College，Liaoning Shenyang 110034，China；2.Department of Geriatrics，the First Affiliated Hospital，China Medical University，Liaoning Shenyang 110001，China；3.Department of Biochemistry and Molecular Biology，College of Basic Medicine，China Medical University，Liaoning Shenyang 110122，China.
Keywords:
celastrol; long non-coding RNA; MEG3; proliferation; apoptosis; hepatocellular carcinoma
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2019.05.006
Abstract:
Objective：To explore the role of MEG3 in the HepG2 cells apoptosis and cells proliferation induced by celastrol.Methods：CCK-8 was used to detect the proliferation of HepG2 cells.Flow cytometry was used to detect HepG2 cells apoptosis.The expressions of cleaved caspase-3，Bax and Bcl-2 protein were detected by Western blot，and real-time PCR was used to detect the expression of MEG3.Results：Celastrol inhibited the proliferation of HepG2 cells and promoted cells apoptosis by up-regulating the expression of MEG3.After knocking down MEG3 with siRNA，it significantly reversed the proliferation inhibition and apoptosis of HepG2 cells induced by celastrol.Conclusion：Celastrol inhibits HepG2 cells proliferation and promotes cell apoptosis by up-regulating MEG3.

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Memo

Memo:
National Natural Science Foundation of China(No.81572831）；国家自然科学基金(编号：81572831)；沈阳市科技计划项目(编号：17-230-9-27)
Last Update: 2019-02-01