|Table of Contents|

Effect of CDK4/6 inhibitor combined with endocrine therapy on miRNA expression in breast cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 05
Page:
723-727
Research Field:
Publishing date:

Info

Title:
Effect of CDK4/6 inhibitor combined with endocrine therapy on miRNA expression in breast cancer cells
Author(s):
Jin Ke1Zhao Dan1Yang Lin2Zhu Jianmei1
1.Oncology Department;2.Traumatology Department,East Department of Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital,Sichuan Chengdu 610000,China.
Keywords:
breast cancerCDK4/6 inhibitorendocrine therapymicroRNAproliferation
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2019.05.003
Abstract:
Objective:To analyze the effect of CDK4/6 inhibitor combined with endocrine drugs on the related miRNA expression in human breast cancer cells.Methods:The human breast cancer cell lines MDA-MB-231,SK-BR-3 and MCF-7 were cultured and treated with palbociclib and anastrozole.MTT was used to detect cell proliferation.BD flow cytometry was used to detect apoptosis and cell cycle.RT-PCR was used to detect the expression of miRNA.Western blot was used to detect the expression of TNF-α and Survivin protein.Results:The expression levels of miRNA-1321,miRNA-574-5p,miRNA-24-3p in the MDA-MB-231 group,SK-BR-3 group and MCF-7 group at 12 h,24 h,48 h and 72 h after drug treatment were lower than control group.But the expression levels of miRNA-1246,miRNA-494-3p and miRNA-29b-3p were significantly higher than those in the control group.The proportions of cells in G0/G1 phase and S phase in MDA-MB-231 group,SK-BR-3 group and MCF-7 group were higher than that in control group,while the ratio of G2/M phase was decreased compared with that in control group.In MDA-MB-231,SK-BR-3 and MCF-7 group,the levels of TNF-α and Survivin were all higher than those in the control group.Conclusion:CDK4/6 inhibitor combined with endocrine drugs can regulate the expression of related miRNAs in human breast cancer cells,thereby inhibiting the proliferation of human breast cancer cells.

References:

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Memo

Memo:
四川省卫生和计划生育委员会科研课题(编号:140082)
Last Update: 2019-02-01