|Table of Contents|

Mechanism of Shan Xian granule-induced apoptosis of H22 liver cancer cells based on Caspase-3/MST1 pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 05
Page:
715-719
Research Field:
Publishing date:

Info

Title:
Mechanism of Shan Xian granule-induced apoptosis of H22 liver cancer cells based on Caspase-3/MST1 pathway
Author(s):
Ying XiaopingXu BingFang YanPan YanfangLi HongJiao PeijuanRui RanDong XiaMeng Bobo
Shaanxi University of Chinese Medicine,Shaanxi Xianyang 712046,China.
Keywords:
Shan Xian granulesCaspase-3MST1
PACS:
R730.52
DOI:
10.3969/j.issn.1672-4992.2019.05.001
Abstract:
Objective:To establish an animal model of H22 hepatocarcinoma xenografts in male Kunming mice and observe the inhibitory effect of Shan Xian granule on H22 liver cancer and its effect on the expression of Caspase-3 and MST1,to explore the effect of Shan Xian granule on the apoptosis of H22 hepatocarcinoma xenografts.Methods:Male Kunming mice were established as H22 hepatoma xenograft tumor models and randomly divided into 5 groups,including blank group,model group,large,medium and small doses of Shan Xian granules groups,administered intragastrically each morning and evening for 30 days.The mice were sacrificed by cervical dislocation.Their tumor was removed and weighed.The tumor suppressor rate was calculated.SABC immunohistochemistry was applied to detect the expression of Caspase-3 and MST1 in transplanted tumor tissues.Results:The tumor inhibition rates of H22 hepatocellular carcinoma in large,medium and small doses of Shan Xian granules were 41.246%,26.801% and 19.835% respectively.The tumor inhibition rate was positively correlated with the dose (P<0.01).Compared with the model group,the expressions of Caspase-3 and MST1 in the large,medium and small doses of Shan Xian granules were significantly enhanced.The expression level was positively correlated with the dose (P<0.01).Conclusion:Shan Xian granules can inhibit the proliferation of H22 hepatocarcinoma xenografts,and its mechanism may be related to the apoptosis of tumor cells induced by the regulation of Caspase-3/MST1 pathway.

References:

[1]Zeng H,Zheng R,Guo Y,et al.Cancer survival in China,2003-2005:A population-based study[J].Int J Cancer,2015,136(8):1921-1930.
[2]An Dexing.Application of anti-tumor Chinese medicine in treatment and prevention of cancer[J].Chinese Med Guide,2017,15(09):16-17.[安德兴.抗肿瘤中药在癌症治疗与预防中的应用[J].中国医药指南,2017,15(09):16-17.]
[3]Dai Xin,Li Zhanquan,Ji Linhua.Research progress of apoptosis related protein caspase[J].Chinese J Modern Med,2010,12(4):130-132.[戴昕,李占全,冀林华.凋亡相关蛋白Caspase研究进展[J].中国现代医药杂志,2010,12(4):130-132.]
[4]Jiao Peijuan,Ying Xiaoping.Research progress on the relationship between caspase-3 and tumor cell apoptosis[J].Shandong Journal of Traditional Chinese Medicine,2017,36(08):721-724.[焦佩娟,应小平.Caspase-3与肿瘤细胞凋亡关系的中医药研究进展[J].山东中医杂志,2017,36(08):721-724.]
[5]Li Shengyou,Ding Yahui,Huang Hongliang.Study of xanthoside A on apoptosis of HepG-2 cells[J].Journal of Guangdong Pharmaceutical University,2018,34(1):50-54.[李胜友,丁亚慧,黄宏靓.旱莲苷A 对HepG-2 细胞凋亡作用的研究[J].广东药科大学学报,2018,34(1):50-54.]
[6]Dong Mengjia,Xu Youqi,Shen Mingqin.Study on the inhibitory effect of Jianpi Huoxue prescription on SMMC-7721 hepatoma in mude mice[J].Chinese Journal of Traditional Chinese Medicine Information,2018,25(2):38-41.[董孟佳,许尤琪,沈明勤.健脾活血方对 SMMC-7721 肝癌裸鼠抑瘤作用的研究[J].中国中医药信息杂志,2018,25(2):38-41.]
[7]Zhu Wenhe,Zhang Wei,Li Yan,et al.Artesunate induces apoptosis of human hepatocellular carcinoma HepG2 cells by increasing the production of reactive oxygen species[J].Chinese Journal of Pathophysiology,2018,34(1):64-69.[朱文赫,张巍,李妍,等.青蒿琥酯通过增加活性氧簇生成诱导人肝癌HepG2 细胞凋亡[J].中国病理生理杂志,2018,34(1):64-69.]
[8]Zhou Jiecan,Tang Shengsong.Research progress on MST1 biological effects and signal regulation[J].Journal of Clinical and Pathology,2016,36(2):209-214.[周杰灿,唐圣松.MST1生物学作用及信号调控研究进展[J].临床与病理杂志,2016,36(2):209-214.]
[9]Lin Xiaoyan,Cui Rongrong,Cai Fengfeng.Protein kinase Mst1/2 and human tumor[J].Chinese General Practice,2016,14(12):2104-2106,2122.[林晓燕,崔嵘嵘,蔡丰丰.蛋白激酶Mst1/2与人类肿瘤[J].中华全科医学,2016,14(12):2104-2106,2122.]
[10]Cao Jun,Jiang Ping,Wang Boyong,et al.Expression of mammalian STE20-related kinase 1 gene in hepatocellular carcinoma and its relationship with hepatitis B virus[J].Chinese J Exp Surg,2016,33(10):2296-2298.[曹军,江平,王波涌,等.哺乳动物STE20相关激酶1基因在肝癌组织的表达及其与乙型肝炎病毒的关系[J].中华实验外科杂志,2016,33(10):2296-2298.]
[11]Liu Deli,Song Qiang,Gao Fang,et al.Expression and significance of mst1 and Mst2 in gastric cancer[J].Modern Oncology,2018,26(12):1863-1867.[刘得利,宋强,高芳,等.MST1和MST2在胃癌中的表达及意义[J].现代肿瘤医学,2018,26(12):1863-1867.]
[12]Zheng Xiaoying,Hao Yuntao,Zhao Shumin,et al.The expression and clinical significance of mst1 protein in cervical cancer[J].Tianjin Medical Journal,2017,45(4):402-405.[郑小影,郝云涛,赵淑敏,等.宫颈癌中 MST1 蛋白的表达及临床意义[J].天津医药,2017,45(4):402-405.]
[13]Zheng Xiaoying,Hao Yuntao,Ma Weijun,et al.Overexpression of mst1 inhibits proliferation,migration and invasion of cervical cancer cell line SiHa[J].Basic and Clin Med,2017,37(3):351-354.[郑小影,郝云涛,马卫军,等.MST1 过表达抑制宫颈癌细胞系 SiHa 的增殖、迁移和侵袭[J].基础医学与临床,2017,37(3):351-354.]
[14]Xu Chuanming.Experimental study on human wild-type Mst1 gene in inhibiting tumor growth[D].Nanchang:Medical School of Nanchang University,2013.[许传铭.人野生型Mst1基因抑制肿瘤生长的实验研究[D].南昌:大学医学院,2013.]
[15]Wen W,Zhu F,Zhang J,et al.MST1 promotes apoptosis through phosphorylation of histone h2AX[J].J Biol Chem,2010,285(50):39108.
[16]Liu ZQ,Lu HF,Yang XH,et al.Effects of MST1 on proliferation and apoptosis of colorectal cancer SW480 cells[J].Journal of Nanchang University (Science Edition),2015,39(6):606-612.[刘卓琦,卢洪飞,杨晓红,等.MST1对结直肠癌SW480细胞增殖与凋亡中的影响[J].南昌大学学报(理科版),2015,39(6):606-612.]
[17]Lin Xiaoyan.Correlation between protein kinase mst1 and apoptosis and prognosis of breast cancer[D].Shandong:Shandong University,2014.[林晓燕.蛋白激酶Mst1与乳腺癌凋亡及预后的相关性[D].山东:山东大学,2014.]
[18]Tian Huanhuan.To explore the mechanism of electroacupuncture "Yishu","Pishu" and "Shenshu" intervening T2DM based on MST1[D].Beijing:Beijing University of Traditional Chinese Medicine,2017.[田环环.基于MST1探究电针“胰俞”、“脾俞”、“肾俞”干预T2DM的机制[D].北京:北京中医药大学,2017.]

Memo

Memo:
National Natural Science Foundation of China(No.81703842);国家自然科学基金项目(编号:81703842);陕西省中管局科研计划项目(编号:JCMS032)
Last Update: 2019-02-01