|Table of Contents|

Expression of WISP1 and Ki67 in gastric cancer and the clinicopathological significance

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 02
Page:
256-261
Research Field:
Publishing date:

Info

Title:
Expression of WISP1 and Ki67 in gastric cancer and the clinicopathological significance
Author(s):
Jiang ShanshanSun DanXin Yan
Department of Laboratory of Gastrointestinal Onco-Pathology,The First Hospital of China Medical University,Liaoning Shenyang 110001,China.
Keywords:
Wnt1-induced signaling pathway protein 1(WISP1)Ki67immunohistochemistrygastric cancer
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2019.02.019
Abstract:
Objective:To investigate the expression of WISP1 and Ki67 in gastric cancer tissues and its clinicopathological significance.Methods:87 gastric cancer samples and 80 gastric mucosal tissue (far away from gastric cancer >5 cm) were collected from patients who underwent resection of primary gastric cancer at The First Hospital of China Medical University,the expression of WISP1 and Ki67 were examined by immunohistochemistry in gastric cancer tissue and normal gastric mucosa tissues.Results:The positive expression rate of WISP1 in gastric cancer tissues was 81.61%(71/87),which was significantly higher than non-cancerous gastric mucosa tissue with positive expression rate of 12.50%(10/80).The expression of WISP1 in poor differentiated adenocarcinoma was significantly higher than well(P=0.032) and mediate(P=0.000) differentiate tubular adenocarcinoma.The positive expression of WISP1 was significant higher in patients with diffuse gastric cancer than intestinal type(P=0.000).The WISP1 expression in patients of gastric cancer with tumor diameter >5 cm was significantly higher than the patients with tumor diameter ≤5 cm(P=0.010).In the tissues of gastric cancer with lymph node metastasis,the positive expression of WISP1 was significantly higher than non-lymph node metastasis(P=0.025).The expression of Ki67 protein was related to the infiltration depth of tumor in patients with advanced gastric cancer(P=0.043) and lymph node metastasis(P=0.027).The relationship between the expression of WISP1 and Ki67 in 87 samples of gastric cancer was positive(rk=0.240,P=0.026).Conclusion:WISP1 expression in gastric cancer tissues was significantly high.The joint examination of WISP1 and Ki67 proteins can better predict lymph node metastasis of gastric cancerand canbe used to evaluate prognosisof gastric cancer patients.

References:

[1] Hashimoto Y,Shindo-Okada N,Tani M,et al.Identification of genes differentially expressed in association with metastatic potential of K-1735 murine melanoma by messenger RNA differential display[J].Cancer Res,1996,56(22):5266-5271.
[2] Xu L,Corcoran RB,Welsh JW,et al.WISP-1 is a Wnt-1- and beta-catenin-responsive oncogene[J].Genes Dev,2000,14(5):585-595.
[3] Su F,Overholtzer M,Besser D,et al.WISP-1 attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase[J].Genes Dev,2002,16(1):46-57.
[4] Pennica D,Swanson TA,Welsh JW,et al.WISP genes are members of the connective tissue growth factor family that are up-regulated in wnt-1-transformed cells and aberrantly expressed in human colon tumors[J].Proc Natl Acad Sci USA,1998,95(25):14717-14722.
[5] Clausen MJ,Melchers LJ,Mastik MF,et al.Identification and validation of WISP1 as an epigenetic regulator of metastasis in oral squamous cell carcinoma[J].Genes Chromosomes Cancer,2016,55(1):45-59.
[6] Nagai Y,Watanabe M,Ishikawa S,et al.Clinical significance of Wnt-induced secreted protein-1 (WISP-1/CCN4) in esophageal squamous cell carcinoma[J].Anticancer Res,2011,31(3):991-997.
[7] Zhang H,Luo H,Hu Z,et al.Targeting WISP1 to sensitize esophageal squamous cell carcinoma to irradiation[J].Oncotarget,2015,6(8):6218-6234.
[8] Yang JY,Yang MW,Huo YM,et al.High expression of WISP-1 correlates with poor prognosis in pancreatic ductal adenocarcinoma[J].Am J Transl Res,2015,7(9):1621-1628.
[9] Gurbuz I,Ferralli J,Roloff T,et al.SAP domain-dependent Mkl1 signaling stimulates proliferation and cell migration by induction of a distinct gene set indicative of poor prognosis in breast cancer patients[J].Mol Cancer,2014,13:22.
[10] Jiang Shanshan,Xin Yan.Research progress of WISP1 and tumors[J].Modern Oncology,2018,26(10):1639-1642.
[11] Chiang KC,Yeh CN,Chung LC,et al.WNT-1 inducible signaling pathway protein-1 enhances growth and tumorigenesis in human breast cancer[J].Sci Rep,2015,5:8686.
[12] Xie D,Nakachi K,Wang H,et al.Elevated levels of connective tissue growth factor,WISP-1,and CYR61 in primary breast cancers associated with more advanced features[J].Cancer Res,2001,61(24):8917-8923.
[13] Wu J,Long Z,Cai H,et al.High expression of WISP1 in colon cancer is associated with apoptosis,invasion and poor prognosis[J].Oncotarget,2016,7(31):49834-49847.
[14] Tian C,Zhou ZG,Meng WJ,et al.Overexpression of connective tissue growth factor WISP-1 in Chinese primary rectal cancer patients[J].World J Gastroenterol,2007,13(28):3878-3882.
[15] Davies SR,Davies ML,Sanders A,et al.Differential expression of the CCN family member WISP-1,WISP-2 and WISP-3 in human colorectal cancer and the prognostic implications[J].Int J Oncol,2010,36(5):1129-1136.
[16] Gerdes J,Lemke H,Baisch H,et al.Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67[J].J Immunol,1984,133(4):1710-1715.
[17] Kloppel G,La Rosa S.Ki67 labeling index:Assessment and prognostic role in gastroenteropancreatic neuroendocrine neoplasms[J].Virchows Arch,2017,472(3):341-349.
[18] Huang G,Chen S,Wang D,et al.High Ki67 expression has prognostic value in surgically-resected T3 gastric adenocarcinoma[J].Clin Lab,2016,62(1-2):141-153.
[19] Min KW,Kim DH,Son BK,et al.A High Ki67/BCL2 index could predict lower disease-free and overall survival in intestinal-type gastric cancer[J].Eur Surg Res,2017,58(3-4):158-168.
[20] Badary DM,Abdel-Wanis ME,Hafez MZ,et al.Immunohistochemical analysis of PTEN,HER2/neu,and ki67 expression in patients with gastric cancer and their association with survival[J].Pathophysiology,2017,24(2):99-106.
[21] Richardsen E,Andersen S,Al-Saad S,et al.Evaluation of the proliferation marker Ki-67 in a large prostatectomy cohort[J].PLoS One,2017,12(11):e186852.

Memo

Memo:
National Natural Science Foundation of China(No.81071650);国家自然科学基金(编号:81071650);辽宁省科技厅科学技术计划项目(编号:2013225585)
Last Update: 1900-01-01