|Table of Contents|

The apoptosis effect of vitamin E succinate on human HER-2 over-expression breast cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 02
Page:
196-201
Research Field:
Publishing date:

Info

Title:
The apoptosis effect of vitamin E succinate on human HER-2 over-expression breast cancer cells
Author(s):
Yu Jing; Jiang Qiuying
The Second Affiliated Hospital of Harbin Medical University，Heilongjiang Harbin 150086,China.
Keywords:
breast cancer; VES; HER-2 over-expression; invasion; migration; apoptosis
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2019.02.004
Abstract:
Objective:To observe the apoptosis effect of vitamin E succinate (VES) on the growth and proliferation of HER-2 over-expression breast cancer cells in vitro.Methods:MTT assay was used to detect the growth inhibition.Western blot was used to screening HER-2 over-expression of breast cancer cell lines and detect the expression satus of GSK-3,NF-κBp65 and caspase 9 in MDA-MB-231 and MDA-MB-453 cell.To observe the migration ability of VES by Transwell assay and Wound-healing for breast cancer cells in vitro,and flow cytometry to detect the effect of VES on apoptosis of breast cancer cells.Results:After treatment,The expression of GSK-3,NF-κBp65 and caspase 9 protein in the breast cancer MDA-MB-453 cells was significantly higher than that in the breast cancer MDA-MB-231 cells.The effect of VES on MDA-MB-231 breast cancer cells significantly enhanced its migration and invasion ability,while inhibited the migration and invasion of MDA-MB-453 cells.VES was used to kill MDA-MB-231 cells directly,and MDA-MB-453 to induce apoptosis.VES had a G1/G0 block in the high expression of breast cancer MDA-MB-453.Conclusion:VES to promote breast cancer cells apoptosis is completed by influencing the multiple signal transduction pathway.VES on different HER-2 over-expression breast cancer cell migration and invasion ability are obviously different,and its mechanism is related to the cell surface protein expression.

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Memo:
黑龙江省自然科学基金项目（编号：D201223）
Last Update: 1900-01-01