|Table of Contents|

Effect of curcumin on biological behavior of human thyroid carcinoma B-CPAP cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 02
Page:
183-187
Research Field:
Publishing date:

Info

Title:
Effect of curcumin on biological behavior of human thyroid carcinoma B-CPAP cells
Author(s):
Sun Wei
Department of General Surgery,the Zhumadian Central Hospital，Henan Zhumadian 463000,China.
Keywords:
curcumin; thyroid carcinoma; B-CPAP cells; apoptosis induction
PACS:
R736.1
DOI:
10.3969/j.issn.1672-4992.2019.02.001
Abstract:
Objective:To investigate the effect of curcumin on the growth and apoptosis of human thyroid carcinoma B-CPAP cells and its possible mechanism.Methods:The human thyroid carcinoma B-CPAP cells were cultured in medium containing different concentrations (10 μmol/L,20 μmol/L,40 μmol/L) of curcumin for 12 h,24 h,36 h,48 h,72 h and 96 h.The effect of curcumin on the growth of B-CPAP cells was observed by MTT.The human thyroid carcinoma B-CPAP cells were cultured in medium containing different concentrations of curcumin for 36 h,48 h,72 h and 96 h.The effects of curcumin in inducing apoptosis of B-CPAP cells were evaluated with Annexin V/PI staining,and the effect of curcumin on ERK pathway,Bax and Bcl-2 expression was detected by Western blot.Results:The growth inhibition rate and apoptosis rate of human thyroid carcinoma B-CPAP cells cultured in medium containing 10 μmol/L,20 μmol/L and 40 μmol/L of curcumin increased gradually with the prolongation of culture time (P＜0.05).With the increase of concentration,the growth inhibition rate and the apoptosis rate also increased gradually at the same time point (P＜0.05).After 72 h of culture,the expression levels of H-Ras,p-Raf-B,p-ERK1/2 and Bcl-2 proteins in B-CPAP cells were significantly lower,while the expression level of Bax protein was significantly higher than that in the blank control group (P＜0.05).Conclusion:Curcumin can inhibit the growth and induce apoptosis of human thyroid carcinoma B-CPAP cells.The mechanism may be related to inhibiting the expression of H-Ras protein and ERK signaling pathway,up-regulating the expression of Bax and down regulating the expression of Bcl-2.

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Memo:
河南省卫生和计划委员会基础研究项目（编号：JH201703054）
Last Update: 1900-01-01