|Table of Contents|

Genetic polymorphism of GSTP1，MTHFR correlated with response to cisplatin and fluorouracil chemotherapy in esophagus cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2016 06
Page:
906-909
Research Field:
Publishing date:

Info

Title:
Genetic polymorphism of GSTP1，MTHFR correlated with response to cisplatin and fluorouracil chemotherapy in esophagus cancer
Author(s):
Gui Yan; Xie Li; Shu Xiaohong; Tan Bangxian; Jiang Lin; Hou Min
Department of Oncology,Affiliated Hospital of North Sichuan Medical College,Sichuan Nanchong 637000,China.
Keywords:
esophageal; glutathione S-transferase Pi-1; methylenetetrahydrofolate reductase; gene polymorphism; cisplatin; 5-fluorouracil; chemotherapy effect
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2016.06.017
Abstract:
Objective:To detect the polymorphism of GSTP1,MTHFR distribution frequency,and investigate the association of the genetic polymorphism of GSTP1,MTHFR with response to cisplatin and fluorouracil chemotherapy in Esophagus cancer.Methods:To select 168 of esophageal cancer patients who underwent PF (cisplatin plus 5 - fluorouracil) chemotherapy to test peripheral blood DNA,gene fragment amplified by PCR method.By using direct sequencing method to examine GSTP1 and MTHFR gene polymorphism.Results:In 168 esophageal cancer patients treated with chemotherapy,curative effect of PF scheme (solid tumor curative effect evaluation standard RECIST 3.0),CR 19 cases(11.3%),PR 60 cases(35.7%),SD 48 cases(28.6%),PD 41 cases(24.4).In esophageal GSTP1 wild type gene patients with（AA） effective rate was 22.0%,hybrid type（AG）+homozygous type（GG） effective rate 25.0%.MTHFR C667T wild type gene（CC）effective rate was 14.3%,the hybrid type（CT）+（TT）homozygous type effective rate was 32.7%,MTHFR A1298C wild type gene（AA） effective rate was 17.3%,hybrid type（AC）+homozygous type（CC） effective rate was 29.8%,including GSTP1 wild-type genotype（AA） and hybrid（AG）+（GG）homozygous type OR=2.520,95%CI:11.237~25.191,P=0.012,MTHFR C667T wild-type genotype（CC） and hybrid（CT）+（TT）homozygous type OR=1.933,95%CI:5.987~16.354,P=0.032,MTHFR A1298C wild-type genotype（AA）and hybrid type（AC）+homozygous CC OR=3.514,95%CI:19.018~27.332,P= 0.511.Results showed that patients with esophageal GSTP1 gene and MTHFR C667T gene polymorphism was associated with PF scheme after chemotherapy curative effect,and hybrid type and homozygous type curative effect was better than the wild type.Conclusion:GSTP1 and MTHFR gene C677T polymorphism associated with the curative effect of 5-fluorouracil.MTHFR A1298C genetic polymorphisms and the curative effect of 5 - fluorouracil has no obvious correlation,so the detection of GSTP1 and MTHFR gene C677T polymorphism - may predict PF chemotherapy curative effect.

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