|Table of Contents|

Establishment and biological characteristics of the cell line of mouse melanoma B16F10-Red

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2016 06
Page:
861-864
Research Field:
Publishing date:

Info

Title:
Establishment and biological characteristics of the cell line of mouse melanoma B16F10-Red
Author(s):
Wu Lingli1; Sun Yongqiang2; Wang Jianhong3; He Xiangfeng3; Zhou Yuqing3; Zheng Liyun3; Zhao Wenjing3; Chen Yuan3; Li Guoxing3; Shi Wen2
1.Department of Clinical Laboratory,Affiliated Zhongda Hospital,Southeast University,Jiangsu Nanjing 210009,China;2.Department of General Surgery,Second Affiliated Hospital,Nantong University,Jiangsu Nantong 226001,China;3.Department of Medicine,Nantong Cancer Hospital Affilated to Nantong University,Jiangsu Nantong 226361,China.
Keywords:
melanoma; B16F10; red fluorescent protein; tumor transplantation
PACS:
R739.5
DOI:
10.3969/j.issn.1672-4992.2016.06.004
Abstract:
Objective:To build expression discosomasp red fluorescent protein mouse melanoma tumor B16F10-Red cell lines and detect its biological characteristics.Methods:DsRed plasmids were introduced into mouse melanoma B16F10 cells transfected with GenEscortTM Ⅱ reagents,G418 pressure cultured the joint limiting dilution method to establish a stable,high-level expression of RFP monoclonal cell lines.FCM was used to detect B16F10 and B16F10-Red cell cycle.B16F10-Red cells colony forming ability was compared with B16F10 cells and mice in vivo tumorigenicity force.Results:The stable expression of RFP mouse melanoma cell line B16F10-Red substantially maintained its parental cell characteristics,can form tumors in C57BL/6 mice subcutaneous abdominal and continue growth and metastasis.Conclution:The B16F10-Red cell lines were successfully constructed,its rate of tumor formation and metastasis xenograft model was no significant difference compared with B16F10 tumor.

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Memo

Memo:
南通市卫生局青年基金项目（编号:WQ2014012）；南通市应用研究计划项目（编号:BK2012067）；南通市社会事业科技创新与示范计划项目（编号:HS2013031）
Last Update: 2016-01-29